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Table 1 Haplotype characteristics and levels of intra- and interspecific diversity of reference DNA sequences

From: DNA barcoding and molecular identification of field-collected Culicoides larvae in the Niayes area of Senegal

Taxon

n

n hap

Intraspecific

H

Interspecific

C. austeni

1

1

0.2

C. bolitinos

13

9

0–0.006 (0.001)

0.91

0.075–0.087 (0.077)

C. brucei

3

3

0.002–0.018 (0.007)

1

0.134–0.145 (0.141)

C. candolfii

2

1

0

0

0.188

C. distinctipennis

7

5

0–0.043 (0.012)

0.904

0.124–0.132 (0.128)

C. dubitatus

3

2

0–0.002 (0.001)

0.666

0.156–0.159 (0.157)

C. enderleini

21

20

0.002–0.05 (0.01)

0.995

0.104–0.12 (0.109)

C. engubandei

2

1

0

0

0.148

C. grahamii

3

3

0.022–0.031 (0.025)

1

0.166–0.180 (0.171)

C. gulbenkiani

3

3

0.004–0.009 (0.006)

1

0.161

C. imicola

17

13

0–0.004 (0.002)

0.963

0.101–0.114 (0.107)

C. isioloensis

2

2

0.002

1

0.163–0.166 (0.164)

C. kanagai

1

1

0.174

C. kibatiensis

1

1

0.153

C. kingi

7

6

0.004–0.009 (0.005)

0.952

0.092–0.097 (0.094)

C. kwagga

2

1

0

0

0.099

C. leucostictus

3

3

0.004–0.011 (0.007)

1

0.124–0.127 (0.126)

C. loxodontis

2

2

0.006

1

0.101–0.109 (0.105)

C. macintoshi

3

3

0.004

1

0.153–0.156 (0.155)

C. magnus

6

3

0–0.007 (0.002)

0.6

0.134–0.142 (0.136)

C. milnei

1

1

0.169

C. miombo

13

4

0–0.002 (0.0005)

0.423

0.114–0.116 (0.114)

C. moreli

6

5

0.004–0.006 (0.003)

0.933

0.193–0.199 (0.195)

C. murphyi

15

6

0–0.002 (0.0004)

0.79

0.142–0.147 (0.144)

C. neavei

2

2

0.099

1

0.161–0.169 (0.165)

C. nevilli

9

9

0.011–0.013 (0.012)

1

0.045–0.164 (0.052)

C. nivosus

5

4

0–0.064 (0.016)

0.9

0.160–0.164 (0.162)

C. ovalis

1

1

0.175

C. oxystoma

14

12

0–0.022 (0.005)

0.978

0.082–0.104 (0.088)

C. pseudopallidipennis

13

8

0–0.009 (0.001)

0.935

0.063–0.072 (0.068)

C. pycnostictus

2

2

0.009

1

0.153–0.156 (0.154)

C. ravus

3

2

0–0.033 (0.011)

0.666

0.132–0.137 (0.135)

C. schultzei

2

2

0.018

1

0.087–0.094 (0.09)

C. similis

7

4

0–0.107 (0.017)

0.809

0.132–0.164 (0.152)

Culicoides sp. #20a

6

5

0–0.004 (0.002)

0.933

0.137–0.145 (0.141)

Culicoides sp. #22a

5

1

0

0

0.101

Culicoides sp. #54a

1

1

0.166

C. subschultzei

6

6

0.004–0.011 (0.006)

1

0.045–0.049 (0.047)

C. tororoensis

2

1

0

0

0.163

C. tropicalis

3

2

0–0.013 (0.004)

0.666

0.127

C. tuttifrutti

4

3

0–0.011 (0.003)

0.833

0.063–0.075 (0.071)

C. zuluensis

8

6

0–0.015 (0.004)

0.928

0.169–0.201 (0.185)

  1. aCulicoides sp. #20, Culicoides sp. #22 and Culicoides sp. #54 are putative new species whose status needs still to be clarified in future taxonomic studies [18]
  2. Abbreviations: n number of cox1 sequences, nhap number of cox1 haplotypes; Intraspecific, range of genetic divergence within taxa (mean), H haplotype diversity values; Interspecific, range of genetic divergence between taxa (mean)