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Table 1 Results of LC-MS/MS analysis of Trichinella britovi adult worms (Ad) selected spots which reacted with pig sera collected at 10 dpi

From: Trichinella britovi muscle larvae and adult worms: stage-specific and common antigens detected by two-dimensional gel electrophoresis-based immunoblotting

Spot

NCBIprot accession no.

MSa

MPb

Seqc

SC (%)d

emPAIe

Mr(kDa)/pIf

Description

1

KRZ04300.1

746

11

10

15

0.82

71.823/6.58

Transketolase, partial

KRY23714.1

363

5

5

8

0.33

75.949/8.54

Succinate dehydrogenase (ubiquinone) flavoprotein subunit, mitochondrial, partial

2

KRY10810.1

817

13

11

16

1.08

70.999/6.60

Transketolase

3

KRY09282.1

1067

18

17

25

1.86

73.168/6.07

Intermediate filament protein ifa-1

KRY23083.1

1015

16

16

22

1.19

76.326/6.17

Stress-70 protein, mitochondrial, partial

4

KRY23083.1

405

6

6

10

0.32

76.326/6.17

Stress-70 protein, mitochondrial, partial

5

KRY23083.1

247

3

3

5

0.18

76.326/6.17

Stress-70 protein, mitochondrial, partial

KRZ06996.1

99

2

2

2

0.12

74.844/5.78

Intermediate filament protein ifa-1, partial

6

KRY11608.1

1889

36

26

40

4.74

75.526/6.24

Intermediate filament protein B

KRY09282.1

1883

31

27

38

4.10

73.168/6.07

Intermediate filament protein ifa-1

KRY16427.1

1772

33

22

22

1.78

108.386/6.14

Heat-shock cognate 71 kDa protein, partial

CAA73574.1

1612

30

21

31

3.15

71.860/5.77

Heat-shock protein 70

7

KRY58599.1

1946

38

26

23

1.54

131.529/6.88

Heat-shock cognate 71 kDa protein, partial

KRY11608.1

1933

37

23

38

5.03

75.526/6.24

Intermediate filament protein B

CAA73574.1

1773

32

25

36

3.87

71.860/5.77

Heat-shock protein 70

KRY09282.1

1503

25

21

29

2.95

73.168/6.07

Intermediate filament protein ifa-1

8

CAA73574.1

1633

35

22

33

3.42

71.860/5.77

Heat-shock protein 70

KRY11608.1

1094

18

17

25

1.62

75.526/6.24

Intermediate filament protein B

KRZ06996.1

906

13

12

17

0.98

74.844/5.78

Intermediate filament protein ifa-1, partial

9

KRY21440.1

646

12

9

16

0.97

62.134/5.04

Calreticulin

KRY19442.1

329

5

5

15

0.81

35.572/9.61

Y-box factor -like protein

10

KRY21426.1

507

8

7

27

1.80

29.821/4.64

Myosin light chain kinase, smooth muscle

AET09716.1

215

3

3

15

0.78

22.620/4.54

Tropomyosin, partial

11

KRZ13803.1

412

6

5

17

1.07

35.565/8.61

32 kDa beta-galactoside-binding lectin lec-3 (Galectin)

12

KRY20423.1

465

8

6

21

1.48

33.995/7.69

32 kDa beta-galactoside-binding lectin (Galectin)

13

KRX13351.1

62

1

1

4

0.15

29.477/8.21

RNA-binding protein rnp-1

14

KRX46812.1

497

7

6

32

2.26

22.941/7.07

Peroxiredoxin-2, partial

15

KRZ77496.1

378

6

4

3

0.08

23.7876/6.48

Dedicator of cytokinesis protein 1

KRY20040.1

140

3

2

10

0.97

18.977/6.97

Heat-shock protein beta-1

16

KRY20040.1

445

12

6

34

3.69

18.977/6.97

Heat-shock protein beta-1

KRX20324.1

323

6

4

28

1.33

19.886/8.12

OV-16 antigen, partial

17

KRY20040.1

405

11

5

30

3.01

18.977/6.97

Heat-shock protein beta-1

KRX20324.1

369

6

4

28

1.43

19.886/8.12

OV-16 antigen, partial

18

KRX47621.1

701

19

9

53

7.94

18.951/6.97

Heat-shock protein beta-1

KRX19442.1

106

2

2

10

0.57

18.490/8.74

Transcription factor BTF3 -like protein 4

19

KRX16844.1

358

11

6

30

2.86

18.894/6.32

Alpha-crystallin B chain

KRY20040.1

297

6

4

26

2.06

18.977/6.97

Heat-shock protein beta-1

KRZ07637.1

278

3

3

14

0.75

23.026/6.12

Stromal cell-derived factor 2

KRZ08373.1

75

2

2

9

0.47

22.324/7.68

Peroxiredoxin-2

20

KRX18074.1

398

8

6

9

0.74

54.209/4.92

BAG family molecular chaperone regulator 2, partial

KRZ17076.1

165

3

2

10

0.75

22.920/5.45

Rho GDP-dissociation inhibitor 1

21

Unidentified

–

–

–

–

–

–

–

22

KRY31449.1

1224

26

15

20

1.11

91.608/5.31

Transitional endoplasmic reticulum ATPase -like protein 2

KRZ08767.1

1011

13

13

7

0.27

234.755/5.91

Myosin-4, partial

KRZ03705.1

287

3

3

4

0.13

101.672/5.38

Paramyosin

KRY00202.1

206

4

3

5

0.20

92.727/5.26

Heat-shock 70 kDa protein 4L

KRY14731.1

172

3

3

3

0.10

131.892/6.63

CAP-Gly domain-containing linker protein 1

23

KRY18882.1

994

15

12

14

0.79

108.605/5.51

LIM domain and actin-binding protein 1

KRZ08767.1

345

6

6

3

0.12

234.755/5.91

Myosin-4, partial

KRZ13693.1

302

5

5

4

0.20

125.148/6.31

Integrin alpha pat-2

24

KRZ06959.1

252

3

3

11

0.61

28.479/7.74

Triosephosphate isomerase, partial

25

KRZ12367.1

299

4

4

24

1.19

24.385/7.01

GTP-binding nuclear protein Ran

26

KRX15368.1

656

14

10

33

3.52

34.025/6.29

32 kDa beta-galactoside-binding lectin, partial

KRY59871.1

115

2

2

12

0.32

30.478/9.08

Serine/arginine-rich splicing factor 1, partial

KRX23478.1

101

2

2

4

0.27

35.530/6.82

Protein MEMO1, partial

27

KRX41818.1

376

5

5

21

1.13

29.431/5.58

Putative phosphomannomutase

KRY00151.1

137

3

2

4

0.25

59.844/5.54

ATP synthase subunit beta, mitochondrial

KRY00848.1

114

2

2

2

0.09

108.425/6.27

Heat-shock 70 kDa protein, partial

28

KRX20997.1

368

6

6

8

0.40

76.565/8.34

Guanine nucleotide-binding proteinalpha-12 subunit, partial

KRX16428.1

294

6

5

23

1.65

26.141/5.80

V-type proton ATPase subunit E

KRZ10894.1

177

3

3

9

0.58

27.709/7.55

GrpE -like protein 1, mitochondrial

29

KRY01036.1

394

8

5

27

2.16

22.274/6.88

Actin-depolymerizing factor 1, isoforms a/b, partial

30

KRY01036.1

440

8

5

27

2.12

22.274/6.88

Actin-depolymerizing factor 1, isoforms a/b, partial

KRY17912.1

295

4

4

18

1.63

17.440/6.18

Uncharacterized protein T12_13420

KRY00151.1

143

4

2

4

0.33

59.844/5.54

ATP synthase subunit beta, mitochondrial

31

KRY01216.1

278

5

5

21

1.85

19885/5.43

Ubiquitin-conjugating enzyme E2 G1, partial

KRY21297.1

199

4

3

14

1.15

21.790/8.89

Peptide methionine sulfoxide reductase MsrB

KRY15966.1

185

4

3

10

0.44

34.259/6.31

Hypothetical protein T12_8663

  1. aMascot score
  2. bMatched peptide
  3. cSequence
  4. dSequence coverage (%)
  5. eExponentially modified protein abundance index
  6. fExperimental nominal mass (kDa) and isoelectric point