Table 4 Results of LC-MS/MS analysis of Trichinella britovi muscle larvae (ML) selected spots which reacted with pig sera collected at 60 dpi
Spot | NCBIprot accession No. | MSa | MPb | Seqc | SC (%)d | emPAIe | Mr(kDa)/pIf | Description |
|---|---|---|---|---|---|---|---|---|
1 | KRX47621.1 | 732 | 22 | 10 | 53 | 13.42 | 18.951/6.97 | Heat-shock protein beta-1 |
KRX19442.1 | 133 | 2 | 2 | 10 | 0.64 | 18.490/8.74 | Transcription factor BTF3 -like protein 4 | |
2 | KRX14469.1 | 241 | 3 | 3 | 2 | 0.06 | 247.333/6.85 | Dedicator of cytokinesis protein 1 |
KRZ13097.1 | 165 | 5 | 3 | 13 | 1.75 | 19.090/5.43 | Heat-shock protein beta-1, partial | |
3 | KRY20040.1 | 376 | 8 | 6 | 34 | 4.92 | 18.977/6.97 | Heat-shock protein beta-1 |
KRX20324.1 | 310 | 5 | 4 | 28 | 1.64 | 19.886/8.12 | OV-16 antigen, partial | |
4 | KRX16844.1 | 662 | 60 | 10 | 66 | 10.33 | 18.894/6.32 | Alpha-crystallin B chain |
KRY20040.1 | 396 | 8 | 7 | 35 | 4.78 | 18.977/6.97 | Heat-shock protein beta-1 | |
KRY18783.1 | 109 | 2 | 2 | 9 | 0.18 | 25.119/6.44 | Stromal cell-derived factor 2 | |
5 | KRX46812.1 | 546 | 11 | 8 | 41 | 4.02 | 22.941/7.07 | Peroxiredoxin-2, partial |
6 | KRX46812.1 | 409 | 8 | 6 | 28 | 2.07 | 22.941/7.07 | Peroxiredoxin-2, partial |
KRZ12367.1 | 292 | 4 | 4 | 19 | 1.02 | 24.385/7.01 | GTP-binding nuclear protein Ran | |
KRX18658.1 | 226 | 3 | 3 | 15 | 0.73 | 23.516/6.92 | ATP synthase subunit d, mitochondrial | |
7 | Unidentified | – | – | – | – | – | – | – |
8 | Unidentified | – | – | – | – | – | – | – |
9 | KRZ13803.1 | 406 | 7 | 5 | 17 | 1.11 | 35.565/8.61 | 32 kDa beta-galactoside-binding lectin lec-3 (Galectin) |
10 | KRZ13803.1 | 584 | 9 | 7 | 24 | 1.58 | 35.565/8.61 | 32 kDa beta-galactoside-binding lectin lec-3 (Galectin) |
KRY30017.1 | 304 | 6 | 5 | 17 | 0.82 | 34.995/8.74 | Putative 3-hydroxyacyl-CoA dehydrogenase | |
11 | KRZ13161.1 | 105 | 2 | 2 | 6 | 0.23 | 42.112/7.12 | Glutamine synthetase |
12 | KRY11984.1 | 432 | 7 | 7 | 15 | 0.90 | 49.560/6.59 | Poly-cysteine and histidine-tailed protein |
KRX28313.1 | 364 | 7 | 6 | 14 | 1.01 | 45.667/6.09 | Calponin -like protein OV9M, partial | |
KRX47308.1 | 240 | 3 | 3 | 3 | 0.14 | 107.151/6.52 | Deoxyribonuclease-2-alpha | |
13 | KRY01407.1 | 324 | 4 | 4 | 10 | 0.46 | 51.099/5.91 | Cuticlin-1, partial |
14 | KRY01407.1 | 319 | 4 | 4 | 10 | 0.48 | 51.099/5.91 | Cuticlin-1, partial |
KRY00848.1 | 166 | 3 | 3 | 2 | 0.15 | 108.425/6.27 | Heat-shock 70 kDa protein, partial | |
15 | CBX25713.1 | 322 | 5 | 5 | 14 | 1.14 | 32.896/4.65 | Tropomyosin, partial |
16 | KRY09099.1 | 476 | 8 | 8 | 22 | 1.47 | 38.218/5.20 | Hypothetical protein T12_13379, partial |
KRX15676.1 | 174 | 3 | 3 | 7 | 0.43 | 35.904/5.46 | 40S ribosomal protein SA, partial | |
17 | KRY09099.1 | 381 | 6 | 6 | 16 | 1.20 | 38.218/5.20 | Hypothetical protein T12_13379, partial |
KRZ15717.1 | 217 | 4 | 3 | 9 | 0.46 | 39.852/5.63 | Guanine nucleotide-binding protein subunit beta-1, partial | |
KRY18502.1 | 203 | 4 | 3 | 3 | 0.26 | 65.700/4.95 | Microtubule-associated protein RP/EB family member 3, partial | |
KRX15059.1 | 126 | 3 | 2 | 5 | 0.52 | 36.189/5.00 | Disorganized muscle protein 1 | |
18 | KRX21567.1 | 504 | 11 | 6 | 21 | 1.66 | 40.427/5.49 | Pyruvate dehydrogenase E1 component subunit beta, mitochondrial |
19 | KRX15059.1 | 667 | 23 | 9 | 28 | 2.96 | 36.189/5.00 | Disorganized muscle protein 1 |
KRZ03570.1 | 403 | 6 | 6 | 18 | 1.20 | 34.457/4.75 | Tropomyosin | |
20 | XP_003378934.1 | 1001 | 20 | 12 | 46 | 6.93 | 28.294/4.83 | 14-3-3 protein zeta |
AET09716.1 | 248 | 4 | 4 | 19 | 1.09 | 22.620/4.54 | Tropomyosin, partial | |
KRX19348.1 | 159 | 3 | 3 | 13 | 0.56 | 28.034/4.82 | Toll-interacting protein | |
21 | KRZ50222.1 | 917 | 16 | 14 | 3 | 0.17 | 449.723/6.87 | Propionyl-CoA carboxylase alpha chain, mitochondrial |
22 | KRZ50222.1 | 1081 | 18 | 17 | 4 | 0.19 | 449.723/6.87 | Propionyl-CoA carboxylase alpha chain, mitochondrial |
23 | KRY09873.1 | 557 | 9 | 9 | 2 | 0.09 | 441.173/6.76 | Propionyl-CoA carboxylase alpha chain, mitochondrial |
24 | KRY45949.1 | 1999 | 45 | 29 | 42 | 6.80 | 73.429/6.07 | Intermediate filament protein ifa-1 |
KRY09282.1 | 1720 | 36 | 26 | 36 | 4.01 | 75.526/6524 | Intermediate filament protein B | |
25 | KRX15368.1 | 437 | 7 | 7 | 20 | 1.80 | 34.025/6.29 | 32 kDa beta-galactoside-binding lectin, partial |
KRZ10402.1 | 91 | 2 | 2 | 4 | 0.33 | 35.568/6.67 | Protein MEMO1, partial | |
KRZ78587.1 | 42 | 3 | 1 | 2 | 0.11 | 48.445/5.59 | Secernin-3 | |
26 | KRY07641.1 | 341 | 6 | 5 | 18 | 1.14 | 38.033/6.38 | 1,5-anhydro-D-fructose reductase |
27 | KRY07641.1 | 239 | 4 | 4 | 12 | 0.63 | 38.033/6.38 | 1,5-anhydro-D-fructose reductase |
28 | Unidentified | – | – | – | – | – | – | – |
29 | XP_003373575.1 | 1255 | 39 | 15 | 41 | 4.48 | 42.210/5.30 | Actin-5C |
AET09716.1 | 168 | 2 | 2 | 11 | 0.45 | 22.620/4.54 | Tropomyosin, partial | |
KRY38295.1 | 160 | 3 | 3 | 6 | 0.26 | 54.444/6.39 | Secernin-3 | |
KRZ06996.1 | 232 | 3 | 3 | 4 | 0.19 | 74.844/5.78 | Intermediate filament protein ifa-1, partial | |
30 | KRY50178.1 | 993 | 18 | 15 | 40 | 4.11 | 46.783/5.44 | Hypothetical protein T03_17187 |
XP_003373575.1 | 527 | 9 | 8 | 25 | 1.47 | 42.210/5.30 | Actin-5C | |
KRZ06996.1 | 363 | 6 | 6 | 9 | 0.47 | 74.844/5.78 | Intermediate filament protein ifa-1, partial | |
KRX47705.1 | 293 | 4 | 4 | 3 | 0.14 | 150.442/6.28 | Serine protease 30 | |
KRZ09733.1 | 323 | 5 | 5 | 5 | 0.28 | 96.031/6.00 | Mitochondrial-processing peptidase subunit beta, partial | |
KRZ17128.1 | 256 | 4 | 4 | 9 | 0.51 | 46.607/5.26 | Putative histone-binding protein Caf1 | |
KRY13378.1 | 250 | 5 | 5 | 9 | 0.54 | 54.969/5.66 | Rab GDP dissociation inhibitor alpha |