Transcriptomic insights into the early host-pathogen interaction of cat intestine with Toxoplasma gondii

Wang, Meng; Zhang, Fu-Kai; Elsheikha, Hany M.; Zhang, Nian-Zhang; He, Jun-Jun; Luo, Jian-Xun; Zhu, Xing-Quan

doi:10.1186/s13071-018-3179-8

Parasites & Vectors

Table 1 Primers used in the qRT-PCR in the present study

From: Transcriptomic insights into the early host-pathogen interaction of cat intestine with Toxoplasma gondii

Gene	Sequence (5'-3')	Primer length (mer)	Tm (°C)	GC %	Product length (bp)
β-actin	ATTCCACGGCACAGTCAAGG	20	63.39	55	110
β-actin	CACCAGCATCACCCCATTT	19	61.75	52.63	110
RSAD2	CACCAGCGTCAACTACCACT	20	59.97	55	140
RSAD2	AATATTCACCGGCTTCCTGC	20	58.32	50	140
SYCN	AAGACGCGCAAGTTCTCGAC	20	61.28	55	117
SYCN	AAGGCATCAGTAACACCTGCAA	22	60.49	45.45	117
CELA1	ATGCTACGCTTCTTGGTGCT	20	60.04	50	239
CELA1	CGGAAGGTCATTTTGCGGTC	20	59.83	50	239
GKN2	CTTGAAGGTGGTATGCCTGGT	21	60	52.38	214
GKN2	CCTGGATGCGATGTAGCGAT	20	60.04	55	214
TFF2	ATGCGTCATGGAAGTCTCGG	22	62.14	50	198
TFF2	TGACAGTCGTCAACGGACATC	24	61.04	45.83	198
LYPF	TTACACCCGACAAACCCTGA	20	59.24	52.38	132
LYPF	CAGGTCTCCGGCCTTTATTCT	21	60.67	55	132
CYP1A1	TCGATACCTACCCAACCCTG	20	58.22	55	246
CYP1A1	ACTGTGTCAAATCCAGCTCCG	21	60.61	52.38	246
CLPS	TGCTCTGCCAAGACACTCTAT	21	58.82	47.62	222
CLPS	5'CAATCAGACAAGGGAGGTGCT	21	60	52.38	222
CPA2	TGCTCAGTCATCTACCAAGCC	21	59.79	52.38	100
CPA2	ATCGACCTGTGTCCCTCAGT	20	60.25	55	100
PRSS2	TACTTCTGCCGCCATGACTC	20	59.82	55	227
PRSS2	TCACTTGGATGCGAGACTTGT	21	59.66	47.62	227
CPA1	AACGATTTACCAAGCCAGCGG	21	61.55	52.38	210
CPA1	GCCGAAGGACCAGTTCAGTA	20	59.39	55	210
FLNC	AGTGGTGCCACCCTGTAACC	20	62.06	60	181
FLNC	CTCTCGCACTTTCACTGGCT	20	60.32	55	181
FN1	GAACACTAATGTCAACTGCCCA	22	58.85	45.45	125
FN1	TGGACCTTGGCAGAGAATCC	20	59.38	55	125
DES	ATGTCCAAGCCAGACCTCAC	20	59.67	55	240
DES	GGAATCGTTGGTGCCCTTGA	20	60.61	55	240
ACTG2	GTATACTCGGTGCTCAAGCC	20	58.15	55	243
ACTG2	TAGGATCCCTCGCTTGCTCT	20	60.11	55	243
FABP6	CCCACAGCTACCAACGCTAC	20	60.74	60	185
FABP6	ATTCTGCTGGAGAGCATCTTCAAT	24	60.39	41.67	185
MYH11	GCATGCTGCAAGATCGAGAG	20	59.42	55	147
MYH11	CTTGCGTGATGCTTGTGTCC	20	60.11	55	147
FGB	CCCAATCAACCTTCGTGTGC	20	59.76	55	156
FGB	TCCTCACATTCTTTGCCAGACA	22	59.89	45.45	156
TAGLN	AACGGCGTGATTCTGAGCAA	20	60.23	47.2	158
TAGLN	CCACCTGCTCCATTTGCTTG	21	59.78	50	158
ALPI	ACACACCTCATGGGCCTCTT	20	61.14	55	199
ALPI	TCAGTGCCAGATAAGCCCTG	20	59.46	55	199
CNN1	TGGCATCATTCTTTGCGAGTTC	22	59.84	45.45	153
CNN1	TCGAAAATGTCGTGGGGCTT	20	60.25	50	153
LYPD2	TGAGATGCTACACCTGTCACG	21	59.80	52.38	123
LYPD2	AGAAGGGGTACACTATCTCCAAG	23	58.71	47.83	123
pCREB	CCACAACCATCCGTCCTTCT	20	62.26	55	137
pCREB	TGTGTGTGTGTGTGTCGGTATGT	23	62.26	47.83	137
MMP	GACGACGATGAGCTGTGGA	19	61.02	57.89	115
MMP	TGGTGTATTCCTTGCCGTTG	20	61.85	50	115
FABP1	CTGATGAAGGTGCCAAGAACAA	22	62.46	45.45	132
FABP1	GCATTTCCTCACTATTTCCCACTC	24	62.3	45.83	132

Abbreviation: Tm, melting temperature

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ISSN: 1756-3305

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