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Fig. 2 | Parasites & Vectors

Fig. 2

From: A chitinase-like protein from Sarcoptes scabiei as a candidate anti-mite vaccine that contributes to immune protection in rabbits

Fig. 2

SDS-PAGE and western blotting of SsCLP5. Lane M: protein molecular weight markers (in kDa); Lane 1: pet-32a (+) expression vector protein; Lane 2: non-purified rSsCLP5 [soluble protein expressed from Escherichia coli BL21 (DE3)]; Lane 3: purified rSsCLP5; Lane 4: total crude proteins from S. scabiei; Lane 5: purified rSsCLP5 detected by serum (diluted 1:120 with 0.01 M PBS) from a rabbit naturally infested with S. scabiei (experimental group); Lane 6: purified rSsCLP5 detected by serum (diluted 1:120 with 0.01 M PBS) from a rSsCLP5-vaccinated rabbit (positive control); Lane 7: purified rSsCLP5 detected by pre-immune rabbit serum (diluted 1:120 with 0.01 M PBS; negative control); Lane 8: total crude proteins detected with rabbit anti-rSsCLP5 serum (diluted 1:120 with 0.01 M PBS). Samples derived from the same experiment and gels/blots were processed in parallel

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Parasites & Vectors

ISSN: 1756-3305

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