Fig. 4

Clinical symptoms in rabbits before, two and four weeks post-challenge. Series a shows normal hind limbs in rabbits before challenge, series b shows clinical symptoms of hind limbs in rabbits after challenge with scabies mites for two weeks, and series c shows clinical symptoms of hind limbs in rabbits after challenge with scabies mites for four weeks. Key: PBS, immunized with 1 ml 0.01 M PBS each time; Quil A, immunized with 1 ml QuilA saponin at concentration of 1 mg/ml (dissolved in PBS) each time; vector protein, immunized with 100 μg (first time) and 200 μg (second time) purified pET32a(+) vector protein with 1 ml QuilA saponin at concentration of 1 mg/ml (dissolved in PBS); rSsCLP5, rSsCLP5’, and rSsCLP5” refer to the test groups immunized with 100 μg then 200 μg purified rSsCLP5 protein with 1 ml QuilA saponin at concentration of 1 mg/ml (dissolved in PBS) at the first and second immunizations