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Fig. 9 | Parasites & Vectors

Fig. 9

From: A chitinase-like protein from Sarcoptes scabiei as a candidate anti-mite vaccine that contributes to immune protection in rabbits

Fig. 9

Variation of specific IgG antibody levels in sera of immunized rabbits as detected by ELISA. Rabbits were immunized twice. Key: PBS, immunized with 1 ml 0.01 M PBS each time; Quil A, immunized with 1 ml QuilA saponin at concentration of 1 mg/ml (dissolved in PBS) each time; vector protein, immunized with 100 μg (first time) and 200 μg (second time) purified pET32a(+) vector protein with 1 ml QuilA saponin at concentration of 1 mg/ml (dissolved in PBS); rSsCLP5, rSsCLP5’, and rSsCLP5” refer to the test groups immunized with 100 μg then 200 μg purified rSsCLP5 protein with 1 ml QuilA saponin at concentration of 1 mg/ml (dissolved in PBS) at the first and second immunizations. Data points correspond to the mean values; error bars represent the standard error. The groups immunized with the rSsCLP5 had significantly higher IgG levels compared to the control groups (F(5,63) = 37.285, P < 0.0001) from week 1 after first immunization to the end of the experiment

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