Table 1 Tick samples used for the molecular portion of this study and the gene amplifications results. Crosses indicate successful amplification and sequencing of the corresponding molecular marker

A. neumanni

na

Argentina

Cordoba

Dean Funes

AN

30°26'S, 64°20'W

×

×

×

×

×

×

A. parvitarsum

na

Argentina

Salta

45 km E of S. Antonio de los Cobres

AP

24°22'S, 66°42'W

×

×

×

×

×

A. tigrinum

na

Argentina

SDE

Pozo Hondo

SDE_16A

27°10'S, 64°30'W

×

×

×

×

×

A. tigrinum

na

Argentina

SDE

Pozo Hondo

SDE_16B

27°10'S, 64°30'W

×

×

×

×

×

A. triste

I

Argentina

Corrientes

Colonia Pellegrini

CR_15A

29°00'S, 57°30'W

×

×

×

×

×

A. triste

I

INTA 1979

Argentina

BA

Inta Delta

BA_AT3A

34°11'S, 58°50'W

×

×

A. triste

I

INTA 1980

Argentina

BA

Inta Delta

BA_AT3B

34°11'S, 58°50'W

×

A. triste

I

INTA 1981

Argentina

BA

Inta Delta

BA_AT3C

34°11'S, 58°50'W

×

×

A. triste

I

INTA 1982

Argentina

BA

Inta Delta

BA_AT3D

34°11'S, 58°50'W

×

×

A. triste

I

INTA 1983

Argentina

BA

Inta Delta

BA_AT3E

34°11'S, 58°50'W

×

×

A. triste

I

INTA 1984

Argentina

BA

Inta Delta

BA_1A

34°11'S, 58°50'W

×

×

×

×

×

A. triste

I

INTA 1985

Argentina

BA

Inta Delta

BA_1A2

34°11'S, 58°50'W

×

×

×

×

A. triste

I

INTA 1986

Argentina

BA

Inta Delta

BA_1B

34°11'S, 58°50'W

×

×

×

×

×

A. triste

I

INTA 1987

Argentina

BA

Inta Delta

BA_1C

34°11'S, 58°50'W

×

×

×

×

×

A. triste

I

INTA 1988

Argentina

BA

Inta Delta

BA_1D

34°11'S, 58°50'W

×

×

×

A. triste

I

INTA 1989

Argentina

BA

Inta Delta

BA_1E

34°11'S, 58°50'W

×

×

×

A. triste

I

INTA 1990

Argentina

BA

Inta Delta

BA_18A

34°11'S, 58°50'W

×

×

×

×

A. triste

I

INTA 1991

Argentina

BA

Inta Delta

BA_18B

34°11'S, 58°50'W

×

×

×

×

A. triste

I

INTA 1992

Argentina

BA

Inta Delta

BA_18C

34°11'S, 58°50'W

×

×

×

×

A. triste

I

INTA 2096

Argentina

Formosa

Reserva El Bagual

FO_12A

26°10'S, 58°56'W

×

×

×

×

×

×

A. triste

I

INTA 2097

Argentina

Formosa

Reserva El Bagual

FO_12B

26°10'S, 58°56'W

×

×

×

A. triste

I

INTA 2098

Argentina

Formosa

Reserva El Bagual

FO_12C

26°10'S, 58°56'W

×

×

×

A. triste

I

INTA 2099

Argentina

Formosa

Reserva El Bagual

FO_12D

26°10'S, 58°56'W

×

×

×

×

A. triste

I

INTA 2100

Argentina

Formosa

Reserva El Bagual

FO_12E

26°10'S, 58°56'W

×

×

×

×

A. triste

I

Argentina

BA

Zarate

BA_AT1A

34°05'S, 59°00'W

×

×

A. triste

I

Argentina

BA

Zarate

BA_AT1B

34°05'S, 59°00'W

×

×

A. triste

I

Argentina

BA

Zarate

BA_AT1C

34°05'S, 59°00'W

×

×

A. triste

I

Argentina

BA

Zarate

BA_17A

34°05'S, 59°00'W

×

×

×

×

A. triste

I

Argentina

BA

Zarate

BA_17B

34°05'S, 59°00'W

×

×

×

×

×

A. triste

I

Argentina

BA

Zarate

BA_17C

34°05'S, 59°00'W

×

×

×

×

×

A. tigrinum

na

Brazil

Goias

Caldas Novas

GO_11A

17°45'S, 48°38'W

×

×

×

×

A. tigrinum

na

Brazil

Goias

Mineiros

GO_5A

17°34'S, 52°33'W

×

×

×

×

×

A. tigrinum

na

Brazil

Goias

Mineiros

GO_5A2

17°34'S, 52°33'W

×

×

×

×

A. tigrinum

na

Brazil

Goias

Mineiros

GO_5B

17°34'S, 52°33'W

×

×

×

A. tigrinum

na

Brazil

Goias

Mineiros

GO_5C

17°34'S, 52°33'W

×

×

×

×

×

A. tigrinum

na

Brazil

Goias

Mineiros

GO_5D

17°34'S, 52°33'W

×

×

A. tigrinum

na

Brazil

Goias

Mineiros

GO_5E

17°34'S, 52°33'W

×

×

×

A. tigrinum

na

Brazil

Goias

Mineiros

GO_3A1

17°34'S, 52°33'W

×

×

×

×

×

A. triste

I

USNMENT1430164

Brazil

MGS

Agua Clara

MGS_6A

20°14'S, 53°23'W

×

×

×

A. triste

I

USNMENT1430165

Brazil

MGS

Agua Clara

MGS_6A2

20°14'S, 53°23'W

×

×

×

×

×

A. triste

I

USNMENT1430166

Brazil

MGS

Agua Clara

MGS_6B

20°14'S, 53°23'W

×

×

×

×

×

A. triste

I

USNMENT1430167

Brazil

MGS

Agua Clara

MGS_6C

20°14'S, 53°23'W

×

×

×

×

×

A. triste

I

USNMENT1430168

Brazil

MGS

Agua Clara

MGS_6D

20°14'S, 53°23'W

×

×

×

×

×

A. triste

I

USNMENT1430169

Brazil

MGS

Agua Clara

MGS_6E

20°14'S, 53°23'W

×

×

×

×

A. triste

I

USNMENT1430170

Brazil

MGS

Agua Clara

MGS_6F

20°14'S, 53°23'W

×

×

×

A. triste

I

USNMENT1430171

Brazil

MGS

Agua Clara

MGS_6G

20°14'S, 53°23'W

×

×

×

A. triste

I

USNMENT1430172

Brazil

MGS

Agua Clara

MGS_8A

20°14'S, 53°23'W

×

×

×

×

×

A. triste

I

USNMENT1430173

Brazil

MGS

Agua Clara

MGS_8A2

20°14'S, 53°23'W

×

×

×

×

×

A. triste

I

USNMENT1430174

Brazil

MGS

Agua Clara

MGS_8B

20°14'S, 53°23'W

×

×

×

×

×

A. triste

I

USNMENT1430175

Brazil

MGS

Agua Clara

MGS_8C

20°14'S, 53°23'W

×

×

×

×

A. triste

I

USNMENT1430176

Brazil

MGS

Agua Clara

MGS_8D

20°14'S, 53°23'W

×

×

×

A. triste

I

USNMENT1430177

Brazil

MGS

Agua Clara

MGS_8E

20°14'S, 53°23'W

×

×

×

A. triste

I

USNMENT1430178

Brazil

MGS

Agua Clara

MGS_8F

20°14'S, 53°23'W

×

×

×

×

×

A. triste

I

USNMENT1430179

Brazil

MGS

Agua Clara

MGS_8G

20°14'S, 53°23'W

×

×

×

×

A. triste

I

USNMENT1430180

Brazil

MGS

Agua Clara

MGS_8H

20°14'S, 53°23'W

×

×

×

×

A. triste

I

USNMENT1430181

Brazil

MGS

Agua Clara

MGS_8I

20°14'S, 53°23'W

×

×

×

×

A. triste

I

USNMENT1430182

Brazil

MGS

Agua Clara

MGS_8J

20°14'S, 53°23'W

×

×

×

×

A. triste

I

USNMENT1430183

Brazil

MGS

Agua Clara

MGS_8K

20°14'S, 53°23'W

×

×

×

×

A. triste

I

USNMENT1430184

Brazil

MGS

Agua Clara

MGS_8L

20°14'S, 53°23'W

×

×

×

A. triste

I

USNMENT1430185

Brazil

MGS

Agua Clara

MGS_8M

20°14'S, 53°23'W

×

×

×

A. triste

I

USNMENT1430186

Brazil

MGS

Agua Clara

MGS_8N

20°14'S, 53°23'W

×

×

×

×

A. triste

I

USNMENT1430187

Brazil

MGS

Agua Clara

MGS_8O

20°14'S, 53°23'W

×

×

×

A. triste

I

USNMENT1430188

Brazil

MGS

Agua Clara

MGS_8P

20°14'S, 53°23'W

×

×

×

×

A. triste

I

USNMENT1430189

Brazil

MGS

Agua Clara

MGS_8Q

20°14'S, 53°23'W

×

×

×

×

A. triste

I

USNMENT01430159

Brazil

Sao Paulo

Promissão

SP_AT2A

21°32'S, 49°52'W

×

×

A. triste

I

USNMENT01430160

Brazil

Sao Paulo

Promissão

SP_AT2B

21°32'S, 49°52'W

×

×

×

A. triste

I

USNMENT01430161

Brazil

Sao Paulo

Promissão

SP_AT2C

21°32'S, 49°52'W

×

×

A. triste

I

USNMENT01430162

Brazil

Sao Paulo

Promissão

SP_AT2D

21°32'S, 49°52'W

×

A. triste

I

USNMENT01430163

Brazil

Sao Paulo

Promissão

SP_AT2E

21°32'S, 49°52'W

×

A. triste

I

USNMENT01430164

Brazil

Sao Paulo

Promissão

SP_AT2F

21°32'S, 49°52'W

×

×

×

A. triste

I

USNMENT01430165

Brazil

Sao Paulo

Promissão

SP_AT2G

21°32'S, 49°52'W

×

×

A. triste

I

USNMENT01430166

Brazil

Sao Paulo

Promissão

SP_2A

21°32'S, 49°52'W

×

A. triste

I

USNMENT01430167

Brazil

Sao Paulo

Promissão

SP_2B

21°32'S, 49°52'W

×

×

×

A. triste

I

USNMENT01430168

Brazil

Sao Paulo

Promissão

SP_2C

21°32'S, 49°52'W

×

×

×

×

A. triste

I

USNMENT01430169

Brazil

Sao Paulo

Promissão

SP_13A

21°32'S, 49°52'W

×

×

×

×

A. triste

I

USNMENT01430170

Brazil

Sao Paulo

Promissão

SP_13B

21°32'S, 49°52'W

×

×

×

A. triste

I

USNMENT01430171

Brazil

Sao Paulo

Promissão

SP_13C

21°32'S, 49°52'W

×

×

×

A. triste

I

USNMENT01430154

Brazil

MGS

Brasilândia

MG_7A

21°16'S, 51°51'W

×

×

×

×

×

A. triste

I

USNMENT01430155

Brazil

MGS

Brasilândia

MG_7B

21°16'S, 51°51'W

×

×

×

×

×

A. triste

I

USNMENT01430156

Brazil

MGS

Brasilândia

MG_7C

21°16'S, 51°51'W

×

×

×

×

A. maculatum

II

Colombia

Santander

Poima

SR_4A

6°09'N, 73°08'W

×

×

×

A. triste

III

USNMENT01430102

Me×ico

Sonora

Mina Mulatos, Sahuaripa

M×_HO1

28°38'N, 108°44'W

×

×

×

×

A. triste

III

USNMENT01430102

Me×ico

Sonora

Mina Mulatos, Sahuaripa

M×_HO2

28°38'N, 108°44'W

×

×

×

×

A. maculatum

IV

USNMENT01430100

Perú

Ica

Ica

PU_IC10A

14°S, 75°46′W

×

A. maculatum

IV

USNMENT01430101

Peru

Piura

Paimas

PU_PI1

4°37'S, 79°57´W

×

×

×

×

×

A. maculatum

IV

USNMENT01430101

Peru

Piura

Paimas

PU_PI2

4°37'S, 79°57'W

×

×

×

×

×

A. triste

IV

Peru

Tumbes

Pampas de Hospital

PU_TU1

3°41'S, 80°26'W

×

×

×

×

A. triste

IV

Peru

Tumbes

Pampas de Hospital

PU_TU2

3°41'S, 80°26'W

×

×

×

A. triste

IV

Peru

Tumbes

Pampas de Hospital

PU_TU3

3°41'S, 80°26'W

×

×

×

×

×

A. triste

I

Uruguay

Canelones

EMA

CN_UY1

34°44'S, 55°58'W

×

×

×

×

×

A. triste

I

Uruguay

Canelones

EMA

CN_UY2

34°44'S, 55°58'W

×

×

×

×

A. triste

III

USNMENT00865800

USA

Arizona

Atascosa Spring, Santa Cruz Co.

AZ16_108

31°24'N, 111°10'W

×

×

×

×

×

A. triste

III

USNMENT00864570

USA

Arizona

Cave Canyon, Santa Cruz Co.

AZ16_14

31°43'N, 110°47'W

×

×

×

×

×

×

A. triste

III

USNMENT00865805

USA

Arizona

San Pedro Riparian NCA, Cochise Co.

AZ16_23

31°33'N, 110°08'W

×

×

×

×

A. triste

III

USNMENT00865805

USA

Arizona

San Pedro Riparian NCA, Cochise Co.

AZ16_40

31°33'N, 110°08'W

×

×

×

×

×

×

A. triste

III

USNMENT00865805

USA

Arizona

San Pedro Riparian NCA, Cochise Co.

AZ16_121

31°22'N, 110°06'W

×

×

×

×

×

A. triste

III

USNMENT00865801

USA

Arizona

Gardner Canyon, Santa Cruz Co.

AZ16_63

31°42'N, 110°47'W

×

×

×

×

×

×

A. triste

III

USNMENT00865800

USA

Arizona

Sycamore Canyon, Santa Cruz Co.

AZ16_1

31°25'N, 111°11'W

×

×

×

A. triste

III

USNMENT00865800

USA

Arizona

Sycamore Canyon, Santa Cruz Co.

AZ16_4

31°25'N, 111°11'W

×

×

×

×

×

A. maculatum

II

USNMENT1430199

USA

Florida

Broward Co.

FL_24A

26°10'N, 80°51'W

×

×

×

×

×

×

A. maculatum

II

USNMENT1430194

USA

Georgia

Bulloch Co.

GA_19A

32°26'N, 81°51'W

×

×

×

×

×

A. maculatum

II

USNMENT1430195

USA

Georgia

Bulloch Co.

GA_19B

32°26'N, 81°51'W

×

×

A. maculatum

II

USNMENT1430196

USA

Georgia

Bulloch Co.

GA_20A

32°26'N, 81°51'W

×

×

×

×

×

A. maculatum

II

USNMENT1430197

USA

Georgia

Bulloch Co.

GA_21A

32°26'N, 81°51'W

×

×

×

×

×

A. maculatum

II

USNMENT1430198

USA

Georgia

Bulloch Co.

GA_21B

32°26'N, 81°51'W

×

×

×

×

A. maculatum

II

USNMENT1430199

USA

Georgia

Bulloch Co.

GA_21C

32°26'N, 81°51'W

×

×

×

×

×

A. maculatum

II

USNMENT1430200

USA

Georgia

Bulloch Co.

GA_21D

32°26'N, 81°51'W

×

×

×

×

A. maculatum

II

USNMENT1430189

USA

Georgia

Camden Co.

GA_23A

30°58'N, 81°42'W

×

×

×

×

×

A. maculatum

II

USNMENT1430184

USA

Georgia

Monroe Co.

GA_22A

33°02'N, 83°44'W

×

×

×

×

×

×