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Table 1 Tick samples used for the molecular portion of this study and the gene amplifications results. Crosses indicate successful amplification and sequencing of the corresponding molecular marker

From: The Amblyomma maculatum Koch, 1844 (Acari: Ixodidae) group of ticks: phenotypic plasticity or incipient speciation?

Species Mt Voucher ID Country State/ province/ department Locality Code Coordinates 12S 16S DL ITS2 cox1 cox2
A. neumanni na   Argentina Cordoba Dean Funes AN 30°26'S, 64°20'W × × × × × ×
A. parvitarsum na   Argentina Salta 45 km E of S. Antonio de los Cobres AP 24°22'S, 66°42'W × ×   × × ×
A. tigrinum na   Argentina SDE Pozo Hondo SDE_16A 27°10'S, 64°30'W × ×   × × ×
A. tigrinum na   Argentina SDE Pozo Hondo SDE_16B 27°10'S, 64°30'W × × × × ×  
A. triste I   Argentina Corrientes Colonia Pellegrini CR_15A 29°00'S, 57°30'W × × × × ×  
A. triste I INTA 1979 Argentina BA Inta Delta BA_AT3A 34°11'S, 58°50'W ×     ×  
A. triste I INTA 1980 Argentina BA Inta Delta BA_AT3B 34°11'S, 58°50'W      ×  
A. triste I INTA 1981 Argentina BA Inta Delta BA_AT3C 34°11'S, 58°50'W ×     ×  
A. triste I INTA 1982 Argentina BA Inta Delta BA_AT3D 34°11'S, 58°50'W      × ×
A. triste I INTA 1983 Argentina BA Inta Delta BA_AT3E 34°11'S, 58°50'W    ×   ×  
A. triste I INTA 1984 Argentina BA Inta Delta BA_1A 34°11'S, 58°50'W × × × × ×  
A. triste I INTA 1985 Argentina BA Inta Delta BA_1A2 34°11'S, 58°50'W × × ×   ×  
A. triste I INTA 1986 Argentina BA Inta Delta BA_1B 34°11'S, 58°50'W × × × × ×  
A. triste I INTA 1987 Argentina BA Inta Delta BA_1C 34°11'S, 58°50'W × × × × ×  
A. triste I INTA 1988 Argentina BA Inta Delta BA_1D 34°11'S, 58°50'W   ×   × ×  
A. triste I INTA 1989 Argentina BA Inta Delta BA_1E 34°11'S, 58°50'W   ×   × ×  
A. triste I INTA 1990 Argentina BA Inta Delta BA_18A 34°11'S, 58°50'W × × ×   ×  
A. triste I INTA 1991 Argentina BA Inta Delta BA_18B 34°11'S, 58°50'W × × × ×   
A. triste I INTA 1992 Argentina BA Inta Delta BA_18C 34°11'S, 58°50'W × × × ×   
A. triste I INTA 2096 Argentina Formosa Reserva El Bagual FO_12A 26°10'S, 58°56'W × × × × × ×
A. triste I INTA 2097 Argentina Formosa Reserva El Bagual FO_12B 26°10'S, 58°56'W   × ×   ×  
A. triste I INTA 2098 Argentina Formosa Reserva El Bagual FO_12C 26°10'S, 58°56'W   × × ×   
A. triste I INTA 2099 Argentina Formosa Reserva El Bagual FO_12D 26°10'S, 58°56'W × × × ×   
A. triste I INTA 2100 Argentina Formosa Reserva El Bagual FO_12E 26°10'S, 58°56'W × × × ×   
A. triste I   Argentina BA Zarate BA_AT1A 34°05'S, 59°00'W     × ×  
A. triste I   Argentina BA Zarate BA_AT1B 34°05'S, 59°00'W ×     ×  
A. triste I   Argentina BA Zarate BA_AT1C 34°05'S, 59°00'W ×     ×  
A. triste I   Argentina BA Zarate BA_17A 34°05'S, 59°00'W × ×   × ×  
A. triste I   Argentina BA Zarate BA_17B 34°05'S, 59°00'W × × × × ×  
A. triste I   Argentina BA Zarate BA_17C 34°05'S, 59°00'W × × × × ×  
A. tigrinum na   Brazil Goias Caldas Novas GO_11A 17°45'S, 48°38'W × × ×   ×  
A. tigrinum na   Brazil Goias Mineiros GO_5A 17°34'S, 52°33'W × × ×   × ×
A. tigrinum na   Brazil Goias Mineiros GO_5A2 17°34'S, 52°33'W × × ×   ×  
A. tigrinum na   Brazil Goias Mineiros GO_5B 17°34'S, 52°33'W × ×    ×  
A. tigrinum na   Brazil Goias Mineiros GO_5C 17°34'S, 52°33'W × × ×   × ×
A. tigrinum na   Brazil Goias Mineiros GO_5D 17°34'S, 52°33'W   × ×    
A. tigrinum na   Brazil Goias Mineiros GO_5E 17°34'S, 52°33'W × × ×    
A. tigrinum na   Brazil Goias Mineiros GO_3A1 17°34'S, 52°33'W × × × × ×  
A. triste I USNMENT1430164 Brazil MGS Agua Clara MGS_6A 20°14'S, 53°23'W × ×   ×   
A. triste I USNMENT1430165 Brazil MGS Agua Clara MGS_6A2 20°14'S, 53°23'W × × × × ×  
A. triste I USNMENT1430166 Brazil MGS Agua Clara MGS_6B 20°14'S, 53°23'W × × × × ×  
A. triste I USNMENT1430167 Brazil MGS Agua Clara MGS_6C 20°14'S, 53°23'W × × × × ×  
A. triste I USNMENT1430168 Brazil MGS Agua Clara MGS_6D 20°14'S, 53°23'W × × × × ×  
A. triste I USNMENT1430169 Brazil MGS Agua Clara MGS_6E 20°14'S, 53°23'W × × × ×   
A. triste I USNMENT1430170 Brazil MGS Agua Clara MGS_6F 20°14'S, 53°23'W   × ×   ×  
A. triste I USNMENT1430171 Brazil MGS Agua Clara MGS_6G 20°14'S, 53°23'W   × ×   ×  
A. triste I USNMENT1430172 Brazil MGS Agua Clara MGS_8A 20°14'S, 53°23'W × × × × ×  
A. triste I USNMENT1430173 Brazil MGS Agua Clara MGS_8A2 20°14'S, 53°23'W × × × × ×  
A. triste I USNMENT1430174 Brazil MGS Agua Clara MGS_8B 20°14'S, 53°23'W × × × × ×  
A. triste I USNMENT1430175 Brazil MGS Agua Clara MGS_8C 20°14'S, 53°23'W × × × ×   
A. triste I USNMENT1430176 Brazil MGS Agua Clara MGS_8D 20°14'S, 53°23'W   ×   × ×  
A. triste I USNMENT1430177 Brazil MGS Agua Clara MGS_8E 20°14'S, 53°23'W   × × ×   
A. triste I USNMENT1430178 Brazil MGS Agua Clara MGS_8F 20°14'S, 53°23'W × × × × ×  
A. triste I USNMENT1430179 Brazil MGS Agua Clara MGS_8G 20°14'S, 53°23'W × × ×   ×  
A. triste I USNMENT1430180 Brazil MGS Agua Clara MGS_8H 20°14'S, 53°23'W × × ×   ×  
A. triste I USNMENT1430181 Brazil MGS Agua Clara MGS_8I 20°14'S, 53°23'W × × × ×   
A. triste I USNMENT1430182 Brazil MGS Agua Clara MGS_8J 20°14'S, 53°23'W × × × ×   
A. triste I USNMENT1430183 Brazil MGS Agua Clara MGS_8K 20°14'S, 53°23'W × × × ×   
A. triste I USNMENT1430184 Brazil MGS Agua Clara MGS_8L 20°14'S, 53°23'W × × ×    
A. triste I USNMENT1430185 Brazil MGS Agua Clara MGS_8M 20°14'S, 53°23'W × × ×    
A. triste I USNMENT1430186 Brazil MGS Agua Clara MGS_8N 20°14'S, 53°23'W × × × ×   
A. triste I USNMENT1430187 Brazil MGS Agua Clara MGS_8O 20°14'S, 53°23'W × × ×    
A. triste I USNMENT1430188 Brazil MGS Agua Clara MGS_8P 20°14'S, 53°23'W × × × ×   
A. triste I USNMENT1430189 Brazil MGS Agua Clara MGS_8Q 20°14'S, 53°23'W × × × ×   
A. triste I USNMENT01430159 Brazil Sao Paulo Promissão SP_AT2A 21°32'S, 49°52'W ×     ×  
A. triste I USNMENT01430160 Brazil Sao Paulo Promissão SP_AT2B 21°32'S, 49°52'W ×   ×   ×  
A. triste I USNMENT01430161 Brazil Sao Paulo Promissão SP_AT2C 21°32'S, 49°52'W ×   ×    
A. triste I USNMENT01430162 Brazil Sao Paulo Promissão SP_AT2D 21°32'S, 49°52'W ×      
A. triste I USNMENT01430163 Brazil Sao Paulo Promissão SP_AT2E 21°32'S, 49°52'W      ×  
A. triste I USNMENT01430164 Brazil Sao Paulo Promissão SP_AT2F 21°32'S, 49°52'W ×   ×    ×
A. triste I USNMENT01430165 Brazil Sao Paulo Promissão SP_AT2G 21°32'S, 49°52'W ×     ×  
A. triste I USNMENT01430166 Brazil Sao Paulo Promissão SP_2A 21°32'S, 49°52'W   ×     
A. triste I USNMENT01430167 Brazil Sao Paulo Promissão SP_2B 21°32'S, 49°52'W × ×    ×  
A. triste I USNMENT01430168 Brazil Sao Paulo Promissão SP_2C 21°32'S, 49°52'W × × ×    ×
A. triste I USNMENT01430169 Brazil Sao Paulo Promissão SP_13A 21°32'S, 49°52'W × × ×   ×  
A. triste I USNMENT01430170 Brazil Sao Paulo Promissão SP_13B 21°32'S, 49°52'W   × × ×   
A. triste I USNMENT01430171 Brazil Sao Paulo Promissão SP_13C 21°32'S, 49°52'W   × ×   ×  
A. triste I USNMENT01430154 Brazil MGS Brasilândia MG_7A 21°16'S, 51°51'W × × × × ×  
A. triste I USNMENT01430155 Brazil MGS Brasilândia MG_7B 21°16'S, 51°51'W × × × × ×  
A. triste I USNMENT01430156 Brazil MGS Brasilândia MG_7C 21°16'S, 51°51'W   × × × ×  
A. maculatum II   Colombia Santander Poima SR_4A 6°09'N, 73°08'W   × ×   ×  
A. triste III USNMENT01430102 Me×ico Sonora Mina Mulatos, Sahuaripa M×_HO1 28°38'N, 108°44'W × × × ×   
A. triste III USNMENT01430102 Me×ico Sonora Mina Mulatos, Sahuaripa M×_HO2 28°38'N, 108°44'W × × × ×   
A. maculatum IV USNMENT01430100 Perú Ica Ica PU_IC10A 14°S, 75°46′W   ×     
A. maculatum IV USNMENT01430101 Peru Piura Paimas PU_PI1 4°37'S, 79°57´W × × ×   × ×
A. maculatum IV USNMENT01430101 Peru Piura Paimas PU_PI2 4°37'S, 79°57'W × × ×   × ×
A. triste IV   Peru Tumbes Pampas de Hospital PU_TU1 3°41'S, 80°26'W × × ×   ×  
A. triste IV   Peru Tumbes Pampas de Hospital PU_TU2 3°41'S, 80°26'W × × ×    
A. triste IV   Peru Tumbes Pampas de Hospital PU_TU3 3°41'S, 80°26'W × × × × ×  
A. triste I   Uruguay Canelones EMA CN_UY1 34°44'S, 55°58'W × × × × ×  
A. triste I   Uruguay Canelones EMA CN_UY2 34°44'S, 55°58'W × ×   × ×  
A. triste III USNMENT00865800 USA Arizona Atascosa Spring, Santa Cruz Co. AZ16_108 31°24'N, 111°10'W × × × ×   ×
A. triste III USNMENT00864570 USA Arizona Cave Canyon, Santa Cruz Co. AZ16_14 31°43'N, 110°47'W × × × × × ×
A. triste III USNMENT00865805 USA Arizona San Pedro Riparian NCA, Cochise Co. AZ16_23 31°33'N, 110°08'W × × × ×   
A. triste III USNMENT00865805 USA Arizona San Pedro Riparian NCA, Cochise Co. AZ16_40 31°33'N, 110°08'W × × × × × ×
A. triste III USNMENT00865805 USA Arizona San Pedro Riparian NCA, Cochise Co. AZ16_121 31°22'N, 110°06'W × × × × ×  
A. triste III USNMENT00865801 USA Arizona Gardner Canyon, Santa Cruz Co. AZ16_63 31°42'N, 110°47'W × × × × × ×
A. triste III USNMENT00865800 USA Arizona Sycamore Canyon, Santa Cruz Co. AZ16_1 31°25'N, 111°11'W × ×   ×   
A. triste III USNMENT00865800 USA Arizona Sycamore Canyon, Santa Cruz Co. AZ16_4 31°25'N, 111°11'W × × × ×   ×
A. maculatum II USNMENT1430199 USA Florida Broward Co. FL_24A 26°10'N, 80°51'W × × × × × ×
A. maculatum II USNMENT1430194 USA Georgia Bulloch Co. GA_19A 32°26'N, 81°51'W × × × × ×  
A. maculatum II USNMENT1430195 USA Georgia Bulloch Co. GA_19B 32°26'N, 81°51'W ×     ×  
A. maculatum II USNMENT1430196 USA Georgia Bulloch Co. GA_20A 32°26'N, 81°51'W × × × × ×  
A. maculatum II USNMENT1430197 USA Georgia Bulloch Co. GA_21A 32°26'N, 81°51'W × × × × ×  
A. maculatum II USNMENT1430198 USA Georgia Bulloch Co. GA_21B 32°26'N, 81°51'W × × ×   ×  
A. maculatum II USNMENT1430199 USA Georgia Bulloch Co. GA_21C 32°26'N, 81°51'W × × × × ×  
A. maculatum II USNMENT1430200 USA Georgia Bulloch Co. GA_21D 32°26'N, 81°51'W × × ×   ×  
A. maculatum II USNMENT1430189 USA Georgia Camden Co. GA_23A 30°58'N, 81°42'W × × × × ×  
A. maculatum II USNMENT1430184 USA Georgia Monroe Co. GA_22A 33°02'N, 83°44'W × × × × × ×
  1. Abbreviations: BA Buenos Aires, SDE Santiago del Estero, na not applicable