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Fig. 1 | Parasites & Vectors

Fig. 1

From: Molecular and biochemical characterization of key enzymes in the cysteine and serine metabolic pathways of Acanthamoeba castellanii

Fig. 1

Dot blot analysis of reactivity of mouse polyclonal antibodies to AcCS, AcGDH or AcSPAT. Strips were spotted with extracted crude proteins from trophozoites (5 μg) and with each purified recombinant protein (1 μg). BMSA and Derf 2 were used as negative controls. One strip was stained with Coomassie brilliant blue. Strips 1, 2 and 3 were treated with anti-AcCS, anti-AcGDH and anti-AcSPAT mouse polyclonal serum, respectively. Strip 4 was treated with normal mouse serum as a negative control

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