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Fig. 2 | Parasites & Vectors

Fig. 2

From: Molecular and biochemical characterization of key enzymes in the cysteine and serine metabolic pathways of Acanthamoeba castellanii

Fig. 2

Localization of native CS, DGH and SPAT in Acanthamoeba trophozoites by confocal microscopy. a-d Localisation of AcCS, e-i colocalisation of AcGDH and AcSPAT in trophozoites. a, e Differential interference contrast microscopy. b Staining with a polyclonal antibody against AcCS. c Nuclear staining with propidium iodide. d Merged image of b and c. f Staining with a polyclonal antibody against AcGDH. g Staining with a polyclonal antibody against AcSPAT. h Nuclear staining with DAPI. i Merged image of f, g and h. Scale-bar: 10 μm

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