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Fig. 3 | Parasites & Vectors

Fig. 3

From: Ecological aspects of Phlebotomines (Diptera: Psychodidae) and the transmission of American cutaneous leishmaniasis agents in an Amazonian/ Guianan bordering area

Fig. 3

PCR-RFLP analysis (primers RPOF2/RPOR2, restriction enzymes TspRI/ HgaI) of Leishmania (Viannia) guyanensis isolates from phlebotomine species and an ACL case from the outskirts of the Oiapoque urban area (lower Oyapock River Basin), Amapá State, Brazil, bordering French Guiana, compared with that of the WHO reference strain. The two columns for each sample represent the digestion products of TspRI and HgaI, respectively. Lane 1: molecular weight marker Smart Ladder; Lane 2: IUMB/BR/2015/M31681; Lane 3: IUMB/BR/2016/M32146; Lane 4: IUMB/BR/2016/M32149; Lane 5: IUMB/BR/2016/M32156; Lane 6: IUMB/BR/2016/M32158; Lane 7: IUMB/BR/2016/M321597; Lane 8: IUMB/BR/2016/M32160; Lane 9: MHOM/BR/2017/M32218; Lane 10: L. (V.) guyanensis WHO reference strain (MHOM/BR/1975/M4147)

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