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Fig. 4 | Parasites & Vectors

Fig. 4

From: Ecological aspects of Phlebotomines (Diptera: Psychodidae) and the transmission of American cutaneous leishmaniasis agents in an Amazonian/ Guianan bordering area

Fig. 4

PCR-RFLP analysis (primers RPOF2/RPOR2, restriction enzymes TspRI/ HgaI) of a 615 bp amplified fragment of the RNA polymerase II gene from Leishmania (Viannia) guyanensis DNA from material remaining on glass dissection slides of infected phlebotomine specimens captured in Oiapoque, Amapá Brazil, compared with that of the WHO reference strain. The two columns for each sample represent the digestion products of TspRI and HgaI, respectively. Lane 1: molecular weight marker Smart Ladder; 1 kb; Lane 2: negative sample; Lane 3: Ny. umbratilis-M32153; Lane 4: negative sample; Lane 5: Ev. infraspinosa-M32155; Lane 6: negative control; Lane 7: WHO reference strain (MHOM/BR/1975/M4147)

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