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Table 1 Primers used in the detection of various tick-borne pathogens and control genes

From: Molecular detection of tick-borne pathogens in canine population and Rhipicephalus sanguineus (sensu lato) ticks from southern Metro Manila and Laguna, Philippines

Organism

Target gene

Primer name

Sequence (5'-3')

Annealing T (°C)

Product size (bp)

Reference

Anaplasma/Ehrlichia spp.

groEL

gro607F

GAAGATGCWGTWGGWTGTACKGC

57

664

[35]

gro1294R

AGMGCTTCWCCTTCWACRTCYTC

gro677F

ATTACTCAGAGTGCTTCTCARTG

57

315

gro1121R

TGCATACCRTCAGTYTTTTCAAC

Anaplasma platys

groESL

PLA-HS475F

AAGGCGAAAGAAGCAGTCTTA

63

513

[36]

PLAT-HS1198R

CATAGTCTGAAGTGGAGGAC

Ehrlichia canis

gltA

EcanisFw

TTATCTGTTTGTGTTATATAAGC

53

1372

[37]

EcanisRev

CAGTACCTATGCATATCAATCC

Rickettsia spp.

gltA

CS2d

ATGACCAATGAAAATAATAAT

52

1250

[38]

CSEndr

CTTATACTCTCTATGTACA

RpCs877p

GGGGGCCTGCTCACGGCGG

52

341

RpCs1258n

ATTGCAAAAAGTACAGTGAAC

Babesia/Hepatozoon

18S rRNA

piro18S F1

GGTGAAACTGCGAATGGCTC

55

1500

This study

piro18S R1

AAGTGATAAGGTTCACAAAACTT

piro18S F2

TGGCTCATTACAACAGTTATA

53

piro18S R2

CGGTCCGAATAATTCACC

Babesia canis

18S rRNA

BcanisF

GTTTATTAGTTTGAAACCCGC

59

456

[39]

BcanisR

GAACTCGAAAAAGCCAAACGA

Hepatozoon canis

18S rRNA

HepF

ATACATGAGCAAAATCTCAAC

50

666

[23]

HepR

CTTATTATTCCATGCTGCAG

Tick control

mt-rrs

mt-rrsF

CTGCTCAATGATTTTTTAAATTGCTGTGG

56

460

[40]

mt-rrsR

CCGGTCTGAACTCAGATCAAGTA

Blood control

actin

Actin-F

CGCACCACCGGCATCGTGAT

65

227

[41]

Actin-R

TCCAGGGCCACGTAGCAGAG

  1. Abbreviation: T temperature