Fig. 8

Electron immunocytochemical studies to follow the location of PBH2 in Leishmania major promastigote forms after treatment with H₂O₂. a In cells treated with 25 mM H₂O₂ the gold labels were observed inside the nucleus (N) in clusters close to the nucleolus. Some dispersed gold particles appear also near the kinetoplast (K) and around the flagellar pocket (FP), or around the vacuoles. The arrows point at clusters of gold marks in the nucleus near the nucleolus. b In cells incubated with 100 mM H₂O₂, some marks appear in the nucleus (N) but less than in the previous treatment. The kinetoplast membrane has a group of marks (arrow) (K), and some gold labels are clustered outside the protozoa