Spliceosomal introns in Trichomonas vaginalis revisited

Parasites & Vectors

Table 1 Summary of the PCR validation for the 62 putative introns distributed in 61 protein-coding genes as annotated by TrichDB

Category^a	Gene ID^b	Notes
(A) Functional introns	TVAG_110020, TVAG_390460, TVAG_126240, TVAG_225200, TVAG_087980, TVAG_413420, TVAG_388620, TVAG_176980, TVAG_053820, TVAG_020880, TVAG_110580, TVAG_350500, TVAG_148640, TVAG_460790, TVAG_198230, TVAG_125100, TVAG_085780, TVAG_065500, TVAG_014960	Described by Vanacova et al. [15]
	TVAG_383350	Described by Deng et al. [16]
	TVAG_324910, TVAG_416520, TVAG_134480, TVAG_089630, TVAG_043580, TVAG_306990, TVAG_147850, TVAG_217460, TVAG_242770, TVAG_056030, TVAG_203580	No early references
(B) Non-functional introns	TVAG_355610, TVAG_411060, TVAG_107710, TVAG_593670, TVAG_045310, TVAG_130170, TVAG_193820, TVAG_479870, TVAG_410120, TVAG_337250, TVAG_455320, TVAG_454570, TVAG_288660, TVAG_178900, TVAG_327510, TVAG_037940, TVAG_525530^c
(C) Undetermined introns	TVAG_066220, TVAG_115540, TVAG_249380, TVAG_296070, TVAG_347440, TVAG_442350, TVAG_115550, TVAG_264700, TVAG_368250, TVAG_416890, TVAG_478810, TVAG_432870, TVAG_458560

^aBased on the RT-PCR results (Additional file 3: Figure S1), these introns were categorized as (A) Functional, (B) Non-functional or (C) Undetermined
^bGenes, where introns were predicted to be in the untranslated regions (UTRs) and not in the coding sequences (CDS), are shown in bold
^cThis is the only gene from the list that was claimed to contain 2 introns instead of 1

ISSN: 1756-3305