Fig. 2

Tissue location of Ts31 at various T. spiralis stages by IFT using anti-rTs31 serum. Immunostaining is distributed at stichosome of ML (a) and IL1 (b), cuticle of 3 dpi male (c), and embryos of 3 dpi female (d). The ML incubated with infection serum was used as a positive control (e), and ML incubated with pre-immune serum as negative controls (f). Scale-bars: 100 μm