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Fig. 3 | Parasites & Vectors

Fig. 3

From: Molecular characterization of a 31 kDa protein from Trichinella spiralis and its induced immune protection in BALB/c mice

Fig. 3

Binding between rTs31 and IECs analyzed by Far-Western blotting. a SDS-PAGE of IEC (Lane 1) and C2C12 (Lane 2) lysates; Lane M: protein marker. b Far-Western blotting of IEC proteins bound with rTs31. The IEC proteins were firstly pre-incubated with rTs31 (Lanes 1–3), MBP (Lanes 4–6) or PBS (Lanes 7–9), and subsequently probed with anti-rTs31 serum (Lanes 1, 4 and 7), infection serum (Lanes 2, 5 and 8) or pre-immune serum (Lanes 3, 6 and 9). c Far-Western blotting of C2C12 proteins bound with rTs31. The C2C12 protein (Lanes 1–3) was first pre-incubated with rTs31 and not recognized by anti-rTs31 serum (Lane 1), infection serum (Lane 2) or pre-immune serum (Lane 3). There was no binding between rTs31 and C2C12 protein

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Parasites & Vectors

ISSN: 1756-3305

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