Fig. 5

IIF analysis of rTs31 binding to IECs. The IECs were first co-cultured with rTs31, IIL ES antigens, MBP or PBS. C2C12 cells were also co-cultured with rTs31. After blocking and washing, the IECs and C2C12 was probed by anti-rTs31 serum, infection serum or pre-immune serum, and subsequently stained using anti-mouse IgG-FITC conjugate. Cell nuclei were dyed by propidium iodide (PI) as the red. Scale-bars: 100 μm