Fig. 8
Killing of NBL mediated by ADCC. a-f Morphology of T. spiralis NBL treated by ADCC test. The NBL (a-d) were treated using anti-rTs31 serum and 2 × 105 mouse PECs at 37 °C for different times: a 24 h, b 36 h, c 48 h and d 60 h. e-f NBL treated by infection serum for 48 h (e) and 60 h (f). g NBL treated using pre-immune serum for 60 h. h NBL incubated without PECs for 60 h. i The cytotoxicity was dose-dependent on anti-rTs31 antibodies. j The cytotoxicity had an evelating trend with culture time prolongation. Asterisks indicate significant differences (*P < 0.001) in comparison to cytotoxicity of pre-immune serum. Scale-bars: 50 μm