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Table 2 Results of the morphological study. The order follows the diagnostic value for species identification. Numbers in parentheses give the count of an observed characteristic per total number of examined characteristics (see Additional file 2 for a detailed evaluation)

From: First record and morphological characterization of an established population of Aedes (Hulecoeteomyia) koreicus (Diptera: Culicidae) in Germany

Character

Ae. koreicus

Ae. j. japonicus

Diagnostic value for species identification

 Hind femur

Base entirely pale-scaled (43/43)

Dark subbasal band (30/30)

Diagnostic character

 Hind tarsomere IV

Complete pale basal ring (41/41)

Most often dark (17/29), few pale scales (6/29) or incomplete pale ring (6/29)

Very variable in Ae. j. japonicus

 Subspiracular patch (L + R)

Usually present (81/86), size very variable if present, 1 to 28 scales, mean number and median 10

Usually absent (59/60), maximum 1 scale counted (1/60)

Ae. koreicus can lack this patch, size varies a lot

 Pedicel scales

Usually more pale than dark scales (40/43)

Usually more dark than pale scales (28/30)

Not easily quantifiable, sometimes scales are evenly distributed in both species

 Costa

Usually no pale scales present (36/43), maximum few pale scales present (7/43)

Usually with pale scales (29/30), only once entirely dark

Number of pale scales very variable in Ae. j. japonicus

 Postpronotum

Often no dark scales present (25/43) or only few dark scales (16/43); clear pattern of dark scales only twice

Usually no dark scales present (29/30), maximum of a few dark scales present (1/30)

Usually no or only a few dark scales present in both species but pale scales more whitish and less crescent shaped scales counted in Ae. koreicus

 Fork scales

Usually no pale scales present (40/43), maximum 3 pale scales counted

Usually some pale scales up to 16 present (19/30) or no pale scales present (11/30)

Not easily recognizable, mainly dark in both species

 Hind tarsomere V

Most often dark (29/41), few pale scales (10/41) or incomplete pale ring (2/41)

Entirely dark (29/29)

Usually dark in both species

 Antepronotum

Broad pale scales (43/43)

Broad pale scales (30/30)

No discrimination possible

 Color of scutal stripes

All scales golden-yellow, prescutellar area usually with paler scales (37/43), never silver-white

All scales golden-yellow (29/29)

No diagnostic character

 Length of anterior dorsocentral stripes on scutum

Stripes usually reach the middle of the scutum (40/42) or slightly shorter (2/42)

Stripes reach at least the middle of the scutum (4/29), usually longer (25/29)

No diagnostic character

Tergite pattern

 Basomedian patch

Usually present on segments II-VII (38/41), at least present on segments II-VI (3/41)

Present on segments II–V up to segments II-VII (20/28), only on segment II (1/28) or absent on all segments (7/28)

No diagnostic character, very variable in Ae. j. japonicus

 Size of basomedian patch

Ranges from a few pale scales (11/41), over pale spots (14/41) to clearly visible bands (15/41); once the patch was fused with the basolateral patches

If present, usually only a few pale scales (20/21), maximum a pale spot present (1/21)

No diagnostic character

 Basolateral patch

Present on segments II–VII (43/43)

Present on segments II–VII (30/30)

No discrimination possible

 Tergit VIII

Basolateral patches usually fused (35/42)

Basolateral patches usually not fused (21/28)

No diagnostic character