Skip to main content
Fig. 4 | Parasites & Vectors

Fig. 4

From: Effects of stable ectopic expression of the primary sex determination gene Yob in the mosquito Anopheles gambiae

Fig. 4

Reverse transcription (RT)-PCR analysis showing altered transcription and pre-mRNA splicing in adult females from the An. gambiae transgenic line 23. a Yob transcript levels in head (H), thorax (T) and abdomen (A) of a transgenic female. b Ribosomal protein S7 transcript levels used as a gel loading control. The same RNA samples were used in (a) and (b). c-f RT-PCR analysis of transcription in three randomly selected transgenic females (Lanes 1-3). c Yob transcript levels. d dsx splicing pattern. e fru splicing pattern. f Ribosomal protein S7 transcript levels used as a gel loading control. The same RNA samples were used in (c-f). In each experiment a whole body RNA sample from a wild-type adult male (M) and female (F) were used for comparison. Note additional, longer Yob transcript isoforms resulting from intron retention in a male; Yob transcripts in transgenic females originate from a cDNA clone, thus lack introns. (-) negative control

Back to article page