Fig. 7

a DNA amplification of 833 bp and 309 bp Nix fragments from the head/thorax of male and female pupae and from 12-24 h embryo pools. b RT-PCR amplification of 833 bp and 309 bp Nix fragments from cDNA derived from the abdomens of the same male and female pupae in a, and cDNA from 12-24 h embryo pools. c RT-PCR amplification of 833 bp Nix fragments from cDNA derived from individual embryos processed 2-4 h after deposition. d RT-PCR amplification of 833 bp Nix fragments from cDNA derived from sexed individual fourth-instar larvae (lanes 1,2,6 and 8 are male larvae). The negative control (C-) without cDNA template is indicated. Amplification of a ribosomal 18S fragment was used as a control for DNA/cDNA integrity