Fig. 4
From: A transgenic tool to assess Anopheles mating competitiveness in the field
PluTo transgenic males allow in vivo assessment of male mating success within the female. Following copulation, the tdTomato-labeled mating plug is distinctly visible through the female cuticle. Autofluorescence is also visible through a wide pass GFP filter set. Mating plug autofluorescence decreases over 4 hours following copulation whereas Plugin-tdTomato fluorescence remains visible for up to 8 hours. Exposure times are indicated below. Scale-bar: 100 μm