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Fig. 6 | Parasites & Vectors

Fig. 6

From: NLRP3 inflammasome activation from Kupffer cells is involved in liver fibrosis of Schistosoma japonicum-infected mice via NF-κB

Fig. 6

NLRP3 inflammasome expression in KCs was more involved in liver fibrosis than in HSCs. NLRP3 immunofluorescent co-staining with F4/80 (KCs marker) (top), or with α-SMA (HSCs marker) (bottom) in liver tissues (a) and in primary KCs or HSCs (c) from mice. The quantitative changes were analyzed by Image-Pro Plus 6.0. b The primary KCs and HSCs were isolated from all mice. Morphology of primary KCs and HSCs was observed under a microscope. The mRNA and protein expressions of NLRP3, Caspase I, and IL-1β in primary KCs and HSCs were detected by RT-PCR (e) and western blot (d), respectively. The expressions of NLRP3, Caspase I, and IL-1β protein were quantified. Data are mean ± SEM of 6 mice/group. *, #P < 0.05, **, ##P < 0.01, ***, ###P < 0.001 vs N; a, bP < 0.05, aa, bbP < 0.01, aaa, bbbP < 0.001 vs SJ. c, d, eP < 0.05, cc, dd, eeP < 0.01, dddP < 0.001. Abbreviations: N, control group; SJ, infected group; NS, no statistical significance. Scale-bars: a, 100 μm; b, 200 μm; c, 100 μm

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