Table 1 Antibodies and reagents used for immunohistochemistry and immunofluorescence
Primary antibody | Antibody type | Dilution buffer (dilution) | Company | Secondary antibody | Dilution buffer (dilution) | Blocking buffer | Wash buffer (time × repetitions) |
|---|---|---|---|---|---|---|---|
NLRP3 | Goat vs mouse NLRP3 (polyclonal) | PBS (1:200)/5% BSA in PBS (1:200) | Abcam, Cambridge, UK | Rabbit vs goat HRP-labeled/donkey vs goat FITC- labeled | PBS (1:500)/5% BSA in PBS (1:50) | 3% H2O2and 3% BSA | PBS (5 min × 3) |
Caspase-1 | Rabbit vs mouse Caspase-1 (polyclonal) | PBS (1:200) | Abcam, Cambridge, UK | Goat vs rabbit HRP-labeled | PBS (1:500) | 3% H2O2and 3% BSA | PBS (5 min × 3) |
IL-1β | Goat vs mouse IL-1β (polyclonal) | PBS (1:100) | R&D, Minneapolis, USA | Rabbit vs goat HRP-labeled | PBS (1:500) | 3% H2O2and 3% BSA | PBS (5 min × 3) |
NF-κB | Rabbit vs mouse NF-κB (polyclonal) | 5% BSA in PBS (1:200) | Abcam, Cambridge, UK | Goat vs rabbit FITC-labeled | 5% BSA in PBS (1:50) | 3% H2O2and 3% BSA | PBS (5 min × 3) |
F4/80 | Rabbit vs mouse F4/80 (polyclonal)/ | 5% BSA in PBS (1:100)/ | Bioss, Beijing, China | Goat vs rabbit Cy3-labeled/ | 5% BSA in PBS (1:50) | 3% H2O2and 3% BSA | PBS (5 min × 3) |
Mouse vs mouse F4/80 (monoclonal) | 5% BSA in PBS (1:50) | Santa, California, USA | Goat vs mouse Cy3-labeled | ||||
α-SMA | Mouse vs mouse α-SMA (polyclonal) | 5% BSA in PBS (1:200) | Abcam, Cambridge, UK | Goat vs mouse Cy3-labeled | 5% BSA in PBS (1:50) | 3% H2O2and 3% BSA | PBS (5 min × 3) |