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Table 1 Antibodies and reagents used for immunohistochemistry and immunofluorescence

From: NLRP3 inflammasome activation from Kupffer cells is involved in liver fibrosis of Schistosoma japonicum-infected mice via NF-κB

Primary antibody Antibody type Dilution buffer (dilution) Company Secondary antibody Dilution buffer (dilution) Blocking buffer Wash buffer
(time × repetitions)
NLRP3 Goat vs mouse NLRP3 (polyclonal) PBS (1:200)/5% BSA in PBS (1:200) Abcam, Cambridge, UK Rabbit vs goat HRP-labeled/donkey vs goat FITC- labeled PBS (1:500)/5% BSA in PBS (1:50) 3% H2O2and 3% BSA PBS (5 min × 3)
Caspase-1 Rabbit vs mouse Caspase-1 (polyclonal) PBS (1:200) Abcam, Cambridge, UK Goat vs rabbit HRP-labeled PBS (1:500) 3% H2O2and 3% BSA PBS (5 min × 3)
IL-1β Goat vs mouse IL-1β (polyclonal) PBS (1:100) R&D, Minneapolis, USA Rabbit vs goat HRP-labeled PBS (1:500) 3% H2O2and 3% BSA PBS (5 min × 3)
NF-κB Rabbit vs mouse NF-κB (polyclonal) 5% BSA in PBS (1:200) Abcam, Cambridge, UK Goat vs rabbit FITC-labeled 5% BSA in PBS (1:50) 3% H2O2and 3% BSA PBS (5 min × 3)
F4/80 Rabbit vs mouse F4/80 (polyclonal)/ 5% BSA in PBS (1:100)/ Bioss, Beijing, China Goat vs rabbit Cy3-labeled/ 5% BSA in PBS (1:50) 3% H2O2and 3% BSA PBS (5 min × 3)
Mouse vs mouse F4/80 (monoclonal) 5% BSA in PBS (1:50) Santa, California, USA Goat vs mouse Cy3-labeled
α-SMA Mouse vs mouse α-SMA (polyclonal) 5% BSA in PBS (1:200) Abcam, Cambridge, UK Goat vs mouse Cy3-labeled 5% BSA in PBS (1:50) 3% H2O2and 3% BSA PBS (5 min × 3)
  1. Abbreviations: NLRP3 nod-like receptor protein-3, Caspase 1 cysteinyl aspartate specific proteinase, IL-1β interleukin, NF-κB nuclear factor-κB, F4/80 mouse epidermal growth factor-like module-containing mucin-like hormone receptor-like 1, α-SMA α-smooth muscle actin