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Table 2 Sequence of primers used for quantitative RT-PCR

From: NLRP3 inflammasome activation from Kupffer cells is involved in liver fibrosis of Schistosoma japonicum-infected mice via NF-κB

Gene

Species

Primer sequences (5'-3')

Annealing T (°C)

Length (bp)

GenBank ID

NLRP3

Mouse

F: GACCAGCCAGAGTGGAATGAC

60

237

NM_145827.3

R: CTGCGTGTAGCGACTGTTGAG

Caspase 1

Mouse

F: GATGGCATTAAGAAGGCCCA

60

229

NM_009807.2

R: CCCTATCAGCAGTGGGCATC

IL-1β

Mouse

F: GGGCCTCAAAGGAAAGAATCT

60

195

NM_008361.4

R: GAGGTGCTGATGTACCAGTTGG

α-SMA

Mouse

F: CCACGAAACCACCTATAACAGC

60

236

NM_007392.3

R: GGAAGGTAGACAGCGAAGCC

Collagen I

Mouse

F: CTGACTGGAAGAGCGGAGAG

60

116

NM_007742.4

R: CGGCTGAGTAGGGAACACAC

GAPDH

Mouse

F: TGAAGGGTGGAGCCAAAAG

60

227

NM_008084.3

R: AGTCTTCTGGGTGGCAGTGAT

NLRP3

Human

F: AAGACAGGAATGCCCGTCTG

60

162

NM_004895.4

R: CCATCTTAATGGGACTCACGG

α-SMA

Human

F: CTTGAGAAGAGTTACGAGTTGC

60

141

NM_001613.3

R: GATGCTGTTGTAGGTGGTTTC

Collagen I

Human

F: AAGACAGTGATTGAATACAAAACCAC

60

132

NM_000088.3

R: GGGAGTTTACAGGAAGCAGACAG

GAPDH

Human

F: CATCATCCCTGCCTCTACTGG

60

259

NM_002046.5

R: GTGGGTGTCGCTGTTGAAGTC

  1. Abbreviations: NLRP3 nod-like receptor protein-3, Caspase 1 cysteinyl aspartate specific proteinase, IL-1β interleukin, α-SMA α-smooth muscle actin, GAPDH glyceraldehyde-3-phosphate dehydrogenase, T temperature