The PAGODAS protocol: pediatric assessment group of dengue and Aedes saliva protocol to investigate vector-borne determinants of Aedes-transmitted arboviral infections in Cambodia

Table 2 Schedule of activities

Procedure	Screen and enroll	Dry ± 28 days	Wet ± 28 days	Dry ± 28 days	Wet ± 28 days	Dry ± 28 days	Wet ± 28 days	Febrile sick visit ± 2 days	Convalescent visit Day 7 ± 2 days	Convalescent visit Day 28 ± 4 days
Informed consent	×
Demographics	×
Baseline medical history questionnaire	×
Baseline physical exam	×
Interval exam and questionnaire		×	×	×	×	×	×
Concomitant medication review	×	×	×	×	×	×	×	×	×	×
Sick visit exam (with height and weight) and questionnaire								×
Convalescent exam and questionnaire									×	×
Height and weight	×						×	×
Vital signs	×	×	×	×	×	×	×	×	×	×
Dengue rapid diagnostic test (RDT)								×
RT-PCR multiplex for acute arboviral diagnosis								× (if RDT+)
Sera collection for ELISAs	×	×	×	×	×	×	×	× (if RDT+)	×
Hemoglobin		×
Alpha-thalassemia screening		×
Peripheral blood mononuclear cells	×						×	× (if RDT+)		×
Complete case report forms	×	×	×	×	×	×	×	×	×	×

Note: Dry seasons visits take place every March whereas wet season visit take place every August

ISSN: 1756-3305