Table 2 Top 50 most abundant proteins from the excretory/secretory proteome profile of adult C. daubneyi. Putative proteins were identified by 2D SDS-PAGE and LC MS/MS. The most significant match is shown for each protein spot scoring above the MASCOT score significance threshold of 47 (spot numbers designated with an * indicate the best match was below the threshold). Putative identifications in bold correspond to gene components identified in the top 50 from the transcript analysis. Previous identification in extracellular-like vesicles is indicated if the same or a similar protein has been reported in parasite extracellular-like vesicles [35,36,37,38] or in the ExoCarta database [39]
Spot | Gene ID | Isoform | MASCOT Score | Uniref90 BLASTp annotation | Alternative annotation | UniProt description | Protein superfamily | Organism | Signal peptide | TM domain | ID in exosomes |
|---|---|---|---|---|---|---|---|---|---|---|---|
1 | TR17138|c0_g1 | i1 | 60 | A0A074ZQZ2_9TREM | – | Uncharacterized protein | Calycin superfamily; fatty-acid binding protein (FABP) family | Opisthorchis viverrini | 0 | 0 | – |
2 | TR18162|c0_g1 | i1 | 255 | A0A075AK05_9TREM | – | Uncharacterized protein | Calycin superfamily; fatty-acid binding protein (FABP) family | Opisthorchis viverrini | 0 | 0 | – |
3 | TR18162|c0_g1 | i1 | 177 | A0A075AK05_9TREM | – | Uncharacterized protein | Calycin superfamily; fatty-acid binding protein (FABP) family | Opisthorchis viverrini | 0 | 0 | – |
4 | TR18162|c0_g1 | i1 | 199 | A0A075AK05_9TREM | – | Uncharacterized protein | Calycin superfamily; fatty-acid binding protein (FABP) family | Opisthorchis viverrini | 0 | 0 | – |
5 | TR17982|c0_g1 | i1 | 620 | I2K1A3_DEKBR | – | Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) | Cyclophilin-type PPIase family | Brettanomyces bruxellensis AWRI1499 | 0 | 0 | Yes |
6 | TR18162|c0_g1 | i1 | 280 | A0A075AK05_9TREM | – | Uncharacterized protein | Calycin superfamily; fatty-acid binding protein (FABP) family | Opisthorchis viverrini | 0 | 0 | – |
7* | TR31531|c0_g1 | i1 | 27 | 0 | No annotations | na | na | na | 0 | 0 | – |
8 | TR18162|c0_g1 | i5 | 343 | A0A075AK05_9TREM | – | Uncharacterized protein | Calycin superfamily; fatty-acid binding protein (FABP) family | Opisthorchis viverrini | 0 | 0 | – |
9 | TR17318|c0_g1 | i1 | 52 | Q5DDT1_SCHJA | – | Putative vesicle-associated membrane protein-associated protein A (SJCHGC09425 protein) | 0 | Schistosoma japonicum (blood fluke) | 0 | Yes | Yes |
10 | TR17219|c0_g1 | i1 | 590 | G4V7Z7_SCHMA | – | Malate dehydrogenase (EC 1.1.1.37) | LDH/MDH superfamily, MDH type 2 family | Schistosoma mansoni (blood fluke) | 0 | 0 | Yes |
11 | TR17138|c0_g1 | i1 | 141 | A0A074ZQZ2_9TREM | – | Uncharacterized protein | Calycin superfamily; fatty-acid binding protein (FABP) family | Opisthorchis viverrini | 0 | 0 |  |
12 | TR11982|c0_g1 | i1 | 221 | G4LUJ6_SCHMA | – | Proteasome subunit beta type (EC 3.4.25.1) | Peptidase T1B family | Schistosoma mansoni (blood fluke) | 0 | 0 | Yes |
13 | TR18162|c0_g1 | i1 | 226 | A0A075AK05_9TREM | – | Uncharacterized protein | Calycin superfamily; fatty-acid binding protein (FABP) family | Opisthorchis viverrini | 0 | 0 | – |
14 | TR17138|c0_g1 | i1 | 97 | A0A074ZQZ2_9TREM | – | Uncharacterized protein | Calycin superfamily; fatty-acid binding protein (FABP) family | Opisthorchis viverrini | 0 | 0 | – |
15* | TR14337|c1_g3 | i2 | 44 | 0 | FABP1_FASGI | Fatty acid-binding protein 1 | Calycin superfamily; fatty-acid binding protein (FABP) family | Fasciola gigantica (giant liver fluke) | 0 | 0 | Yes |
16 | TR18374|c0_g1 | i1 | 663 | S4UI50_FASHE | – | Triosephosphate isomerase (EC 5.3.1.1) | Triosephosphate isomerase family | Fasciola hepatica (liver fluke) | 0 | 0 | Yes |
17 | TR22034|c1_g4 | i3 | 761 | A0A075A0M6_9TREM | – | Fructose-bisphosphate aldolase (EC 4.1.2.13) | Class I fructose-bisphosphate aldolase family | Opisthorchis viverrini | 0 | 0 | Yes |
18* | TR31531|c0_g1 | i1 | 26 | 0 | No annotations | na | na | na | 0 | 0 | – |
19 | TR17367|c0_g1 | i1 | 642 | G7YF68_CLOSI | – | Enolase | 0 | Clonorchis sinensis (Chinese liver fluke) | 0 | 0 | Yes |
20* | TR15346|c0_g1 | i1 | 33 | G4VM15_SCHMA | – | Putative eukaryotic translation initiation factor 4b/4h | 0 | Schistosoma mansoni (blood fluke) | 0 | 0 | – |
21 | TR18162|c0_g1 | i1 | 118 | A0A075AK05_9TREM | – | Uncharacterized protein | Calycin superfamily; fatty-acid binding protein (FABP) family | Opisthorchis viverrini | 0 | 0 | – |
22 | TR21279|c0_g5 | i1 | 202 | C1LY00_SCHJA | – | Glutathione S-transferase (EC 2.5.1.18) | 0 | Schistosoma japonicum (blood fluke) | 0 | 0 | Yes |
23* | TR22414|c0_g1 | i1 | 27 | 0 | No annotations | na | na | na | 0 | 0 | – |
24 | TR18162|c0_g1 | i1 | 124 | A0A075AK05_9TREM | – | Uncharacterized protein | Calycin superfamily; fatty-acid binding protein (FABP) family | Opisthorchis viverrini | 0 | 0 | – |
25 | TR43052|c0_g1 | i1 | 53 | 0 | No annotations | na | na | na | 0 | 0 | – |
26 | TR18535|c1_g1 | i1 | 75 | U6P7F0_HAECO | – | Uncharacterized protein | Peptidase C1 family | Haemonchus contortus (Barber pole worm) | 0 | 0 | – |
27 | TR20917|c0_g1 | i1 | 230 | Q4VRW5_9TREM | – | Cathepsin B1 isotype 5 | Peptidase C1 family | Trichobilharzia regenti | Yes | 0 | Yes |
28* | TR23151|c0_g1 | i1 | 29 | G7YVU0_CLOSI | – | ADIPOR-like receptor CG5315 | 0 | Clonorchis sinensis (Chinese liver fluke) | 0 | Yes | – |
29 | TR20917|c0_g1 | i1 | 365 | Q4VRW5_9TREM | – | Cathepsin B1 isotype 5 | Peptidase C1 family | Trichobilharzia regenti | Yes | 0 | Yes |
30 | TR21279|c0_g5 | i1 | 142 | C1LY00_SCHJA | – | Glutathione S-transferase (EC 2.5.1.18) | 0 | Schistosoma japonicum (blood fluke) | 0 | 0 | Yes |
31 | TR20917|c0_g1 | i1 | 255 | Q4VRW5_9TREM | – | Cathepsin B1 isotype 5 | Peptidase C1 family | Trichobilharzia regenti | Yes | 0 | Yes |
32 | TR23254|c0_g1 | i1 | 595 | C6FWH0_CLOSI | – | Leucine aminopeptidase 2 (Leucyl aminopeptidase) | 0 | Clonorchis sinensis (Chinese liver fluke) | 0 | 0 | Yes |
33 | TR13284|c0_g1 | i1 | 74 | F6YCC0_HORSE | – | Uncharacterized protein | 0 | Equus caballus (horse) | Yes | 0 | – |
34 | TR10146|c0_g1 | i1 | 49 | 0 | No annotations | na | na | na | 0 | 0 | – |
35 | TR16703|c0_g1 | i1 | 89 | J9HU03_9SPIT | – | Cystatin B, putative | 0 | Oxytricha trifallax | 0 | Yes | Yes |
36 | TR13284|c0_g1 | i1 | 112 | F6YCC0_HORSE | – | Uncharacterized protein | 0 | Equus caballus (horse) | Yes | 0 | – |
37 | TR23254|c0_g1 | i1 | 576 | C6FWH0_CLOSI | – | Leucine aminopeptidase 2 (Leucyl aminopeptidase) | 0 | Clonorchis sinensis (Chinese liver fluke) | 0 | 0 | Yes |
38 | TR23254|c0_g1 | i1 | 309 | C6FWH0_CLOSI | – | Leucine aminopeptidase 2 (Leucyl aminopeptidase) | 0 | Clonorchis sinensis (Chinese liver fluke) | 0 | 0 | Yes |
39 | TR18926|c0_g1 | i1 | 137 | V4B1M6_LOTGI | – | Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) | Cyclophilin-type PPIase family | Lottia gigantea (giant owl limpet) | 0 | Yes | Yes |
40 | TR17112|c0_g1 | i1 | 299 | Q25595_CLOSI | – | Putative glutathione transferase | GST superfamily | Clonorchis sinensis (Chinese liver fluke) | 0 | 0 | Yes |
41 | TR23254|c0_g1 | i1 | 613 | C6FWH0_CLOSI | – | Leucine aminopeptidase 2 (Leucyl aminopeptidase) | 0 | Clonorchis sinensis (Chinese liver fluke) | 0 | 0 | Yes |
42 | TR18162|c0_g1 | i1 | 105 | A0A075AK05_9TREM | – | Uncharacterized protein | Calycin superfamily; fatty-acid binding protein (FABP) family | Opisthorchis viverrini | 0 | 0 | – |
43 | TR17367|c0_g1 | i1 | 438 | G7YF68_CLOSI | – | Enolase | 0 | Clonorchis sinensis (Chinese liver fluke) | 0 | 0 | Yes |
44 | TR18162|c0_g1 | i1 | 84 | A0A075AK05_9TREM | – | Uncharacterized protein | Calycin superfamily; fatty-acid binding protein (FABP) family | Opisthorchis viverrini | 0 | 0 | – |
45* | TR21653|c0_g1 | i1 | 30 | G4VA64_SCHMA | – | Palmitoyltransferase (EC 2.3.1.225) | DHHC palmitoyltransferase family | Schistosoma mansoni (blood fluke) | 0 | Yes | – |
46 | TR25811|c0_g1 | i1 | 79 | 0 | A0A085M8I9_9BILA | Uncharacterized protein | 0 | Trichuris suis (pig whipworm) | 0 | 0 | – |
47 | TR20361|c12_g1 | i1 | 294 | H2KV75_CLOSI | – | Putative eggshell protein | 0 | Clonorchis sinensis (Chinese liver fluke) | Yes | Yes | – |
48 | TR18535|c1_g1 | i1 | 76 | U6P7F0_HAECO | – | Uncharacterized protein | Peptidase C1 family | Haemonchus contortus (Barber pole worm) | 0 | 0 | – |
49 | TR17982|c0_g1 | i1 | 519 | I2K1A3_DEKBR | – | Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) | Cyclophilin-type PPIase family | Brettanomyces bruxellensis AWRI1499 | 0 | 0 | Yes |
50 | TR16703|c0_g1 | i1 | 54 | J9HU03_9SPIT | – | Cystatin B, putative | 0 | Oxytricha trifallax | 0 | Yes | Yes |