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Table 2 Top 50 most abundant proteins from the excretory/secretory proteome profile of adult C. daubneyi. Putative proteins were identified by 2D SDS-PAGE and LC MS/MS. The most significant match is shown for each protein spot scoring above the MASCOT score significance threshold of 47 (spot numbers designated with an * indicate the best match was below the threshold). Putative identifications in bold correspond to gene components identified in the top 50 from the transcript analysis. Previous identification in extracellular-like vesicles is indicated if the same or a similar protein has been reported in parasite extracellular-like vesicles [35,36,37,38] or in the ExoCarta database [39]

From: Polyomic tools for an emerging livestock parasite, the rumen fluke Calicophoron daubneyi; identifying shifts in rumen functionality

Spot Gene ID Isoform MASCOT Score Uniref90 BLASTp annotation Alternative annotation UniProt description Protein superfamily Organism Signal peptide TM domain ID in exosomes
1 TR17138|c0_g1 i1 60 A0A074ZQZ2_9TREM Uncharacterized protein Calycin superfamily; fatty-acid binding protein (FABP) family Opisthorchis viverrini 0 0
2 TR18162|c0_g1 i1 255 A0A075AK05_9TREM Uncharacterized protein Calycin superfamily; fatty-acid binding protein (FABP) family Opisthorchis viverrini 0 0
3 TR18162|c0_g1 i1 177 A0A075AK05_9TREM Uncharacterized protein Calycin superfamily; fatty-acid binding protein (FABP) family Opisthorchis viverrini 0 0
4 TR18162|c0_g1 i1 199 A0A075AK05_9TREM Uncharacterized protein Calycin superfamily; fatty-acid binding protein (FABP) family Opisthorchis viverrini 0 0
5 TR17982|c0_g1 i1 620 I2K1A3_DEKBR Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) Cyclophilin-type PPIase family Brettanomyces bruxellensis AWRI1499 0 0 Yes
6 TR18162|c0_g1 i1 280 A0A075AK05_9TREM Uncharacterized protein Calycin superfamily; fatty-acid binding protein (FABP) family Opisthorchis viverrini 0 0
7* TR31531|c0_g1 i1 27 0 No annotations na na na 0 0
8 TR18162|c0_g1 i5 343 A0A075AK05_9TREM Uncharacterized protein Calycin superfamily; fatty-acid binding protein (FABP) family Opisthorchis viverrini 0 0
9 TR17318|c0_g1 i1 52 Q5DDT1_SCHJA Putative vesicle-associated membrane protein-associated protein A (SJCHGC09425 protein) 0 Schistosoma japonicum (blood fluke) 0 Yes Yes
10 TR17219|c0_g1 i1 590 G4V7Z7_SCHMA Malate dehydrogenase (EC 1.1.1.37) LDH/MDH superfamily, MDH type 2 family Schistosoma mansoni (blood fluke) 0 0 Yes
11 TR17138|c0_g1 i1 141 A0A074ZQZ2_9TREM Uncharacterized protein Calycin superfamily; fatty-acid binding protein (FABP) family Opisthorchis viverrini 0 0  
12 TR11982|c0_g1 i1 221 G4LUJ6_SCHMA Proteasome subunit beta type (EC 3.4.25.1) Peptidase T1B family Schistosoma mansoni (blood fluke) 0 0 Yes
13 TR18162|c0_g1 i1 226 A0A075AK05_9TREM Uncharacterized protein Calycin superfamily; fatty-acid binding protein (FABP) family Opisthorchis viverrini 0 0
14 TR17138|c0_g1 i1 97 A0A074ZQZ2_9TREM Uncharacterized protein Calycin superfamily; fatty-acid binding protein (FABP) family Opisthorchis viverrini 0 0
15* TR14337|c1_g3 i2 44 0 FABP1_FASGI Fatty acid-binding protein 1 Calycin superfamily; fatty-acid binding protein (FABP) family Fasciola gigantica (giant liver fluke) 0 0 Yes
16 TR18374|c0_g1 i1 663 S4UI50_FASHE Triosephosphate isomerase (EC 5.3.1.1) Triosephosphate isomerase family Fasciola hepatica (liver fluke) 0 0 Yes
17 TR22034|c1_g4 i3 761 A0A075A0M6_9TREM Fructose-bisphosphate aldolase (EC 4.1.2.13) Class I fructose-bisphosphate aldolase family Opisthorchis viverrini 0 0 Yes
18* TR31531|c0_g1 i1 26 0 No annotations na na na 0 0
19 TR17367|c0_g1 i1 642 G7YF68_CLOSI Enolase 0 Clonorchis sinensis (Chinese liver fluke) 0 0 Yes
20* TR15346|c0_g1 i1 33 G4VM15_SCHMA Putative eukaryotic translation initiation factor 4b/4h 0 Schistosoma mansoni (blood fluke) 0 0
21 TR18162|c0_g1 i1 118 A0A075AK05_9TREM Uncharacterized protein Calycin superfamily; fatty-acid binding protein (FABP) family Opisthorchis viverrini 0 0
22 TR21279|c0_g5 i1 202 C1LY00_SCHJA Glutathione S-transferase (EC 2.5.1.18) 0 Schistosoma japonicum (blood fluke) 0 0 Yes
23* TR22414|c0_g1 i1 27 0 No annotations na na na 0 0
24 TR18162|c0_g1 i1 124 A0A075AK05_9TREM Uncharacterized protein Calycin superfamily; fatty-acid binding protein (FABP) family Opisthorchis viverrini 0 0
25 TR43052|c0_g1 i1 53 0 No annotations na na na 0 0
26 TR18535|c1_g1 i1 75 U6P7F0_HAECO Uncharacterized protein Peptidase C1 family Haemonchus contortus (Barber pole worm) 0 0
27 TR20917|c0_g1 i1 230 Q4VRW5_9TREM Cathepsin B1 isotype 5 Peptidase C1 family Trichobilharzia regenti Yes 0 Yes
28* TR23151|c0_g1 i1 29 G7YVU0_CLOSI ADIPOR-like receptor CG5315 0 Clonorchis sinensis (Chinese liver fluke) 0 Yes
29 TR20917|c0_g1 i1 365 Q4VRW5_9TREM Cathepsin B1 isotype 5 Peptidase C1 family Trichobilharzia regenti Yes 0 Yes
30 TR21279|c0_g5 i1 142 C1LY00_SCHJA Glutathione S-transferase (EC 2.5.1.18) 0 Schistosoma japonicum (blood fluke) 0 0 Yes
31 TR20917|c0_g1 i1 255 Q4VRW5_9TREM Cathepsin B1 isotype 5 Peptidase C1 family Trichobilharzia regenti Yes 0 Yes
32 TR23254|c0_g1 i1 595 C6FWH0_CLOSI Leucine aminopeptidase 2 (Leucyl aminopeptidase) 0 Clonorchis sinensis (Chinese liver fluke) 0 0 Yes
33 TR13284|c0_g1 i1 74 F6YCC0_HORSE Uncharacterized protein 0 Equus caballus (horse) Yes 0
34 TR10146|c0_g1 i1 49 0 No annotations na na na 0 0
35 TR16703|c0_g1 i1 89 J9HU03_9SPIT Cystatin B, putative 0 Oxytricha trifallax 0 Yes Yes
36 TR13284|c0_g1 i1 112 F6YCC0_HORSE Uncharacterized protein 0 Equus caballus (horse) Yes 0
37 TR23254|c0_g1 i1 576 C6FWH0_CLOSI Leucine aminopeptidase 2 (Leucyl aminopeptidase) 0 Clonorchis sinensis (Chinese liver fluke) 0 0 Yes
38 TR23254|c0_g1 i1 309 C6FWH0_CLOSI Leucine aminopeptidase 2 (Leucyl aminopeptidase) 0 Clonorchis sinensis (Chinese liver fluke) 0 0 Yes
39 TR18926|c0_g1 i1 137 V4B1M6_LOTGI Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) Cyclophilin-type PPIase family Lottia gigantea (giant owl limpet) 0 Yes Yes
40 TR17112|c0_g1 i1 299 Q25595_CLOSI Putative glutathione transferase GST superfamily Clonorchis sinensis (Chinese liver fluke) 0 0 Yes
41 TR23254|c0_g1 i1 613 C6FWH0_CLOSI Leucine aminopeptidase 2 (Leucyl aminopeptidase) 0 Clonorchis sinensis (Chinese liver fluke) 0 0 Yes
42 TR18162|c0_g1 i1 105 A0A075AK05_9TREM Uncharacterized protein Calycin superfamily; fatty-acid binding protein (FABP) family Opisthorchis viverrini 0 0
43 TR17367|c0_g1 i1 438 G7YF68_CLOSI Enolase 0 Clonorchis sinensis (Chinese liver fluke) 0 0 Yes
44 TR18162|c0_g1 i1 84 A0A075AK05_9TREM Uncharacterized protein Calycin superfamily; fatty-acid binding protein (FABP) family Opisthorchis viverrini 0 0
45* TR21653|c0_g1 i1 30 G4VA64_SCHMA Palmitoyltransferase (EC 2.3.1.225) DHHC palmitoyltransferase family Schistosoma mansoni (blood fluke) 0 Yes
46 TR25811|c0_g1 i1 79 0 A0A085M8I9_9BILA Uncharacterized protein 0 Trichuris suis (pig whipworm) 0 0
47 TR20361|c12_g1 i1 294 H2KV75_CLOSI Putative eggshell protein 0 Clonorchis sinensis (Chinese liver fluke) Yes Yes
48 TR18535|c1_g1 i1 76 U6P7F0_HAECO Uncharacterized protein Peptidase C1 family Haemonchus contortus (Barber pole worm) 0 0
49 TR17982|c0_g1 i1 519 I2K1A3_DEKBR Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) Cyclophilin-type PPIase family Brettanomyces bruxellensis AWRI1499 0 0 Yes
50 TR16703|c0_g1 i1 54 J9HU03_9SPIT Cystatin B, putative 0 Oxytricha trifallax 0 Yes Yes
  1. Abbreviation: na not applicable