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Fig. 3 | Parasites & Vectors

Fig. 3

From: Evaluation of rapid extraction and isothermal amplification techniques for the detection of Leishmania donovani DNA from skin lesions of suspected cases at the point of need in Sri Lanka

Fig. 3

Selection of the best target sample for the RPA assay. Results of screening the skin punch biopsy, scraping and fine needle aspirates from patient numbers 4 and 5 to determine which sample would give the best results with SpeedXtract and RPA at the point of need. The 2 mm punch biopsy sample of patient 5 produced the best exponential amplification curve and early fluorescence signal in RPA assay. All samples from patient 4 were negative. The graph was produced by T8 desktop software (Axxin, Fairfield, Australia)

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