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Fig. 1 | Parasites & Vectors

Fig. 1

From: Combination of microbiome analysis and serodiagnostics to assess the risk of pathogen transmission by ticks to humans and animals in central Germany

Fig. 1

Workflow of all samples. DNA of ticks and animal blood samples was extracted and PCRs for Bartonella-specific genes (16S rDNA, 16S-23S ITS) were conducted (with subsequent Sanger-sequencing of the amplicons). 16S rDNA metagenomics was used for determination of the tick microbiome (confirmed by specific PCRs) revealing the presence of further pathogens. Serum of pets and, if available, of pet owners was analyzed for serological infection markers (antibodies) known to indicate previous infections in regard to the molecular findings from ticks

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