Fig. 1

Alignment of amino acid sequences and phylogenetic analysis of TRAPs. a Graphic depiction of BoTRAP1. A signal peptide at the N-terminus, two functional domains (vWFA and TSP-1), a transmembrane region and a cytoplasmic C-terminal tail domain were dispersed in the coding region. b BoTRAP1 sequence alignment across various strains of apicomplexan parasites, and the sequences were aligned using ClustalW. c Neighbor-joining tree showing phylogenetic relationship of the BoTRAP1 sequences of two conserved regions identified in this study, with sequences of other apicomplexan parasite. The scale-bar represents the nucleotide substitutions per position. Branch lengths represent the amount of genetic distance change between the strains. Abbreviations: SP, signal peptide; TR, transmembrane region; CTD, C-terminal tail domain