Table 1 Monoclonal antibodies used in flow cytometry analysis of peritoneal cavity cell populations
Peritoneal cell immunophenotypes | Antibodies | Fluorochromes | Clones | Reference |
|---|---|---|---|---|
Isotype controls | ||||
Control FITC | mouse control IgG1 | FITC | – | MCA928F |
Control RPE | mouse control IgG2a | RPE | – | MCA929PE |
Cell populations | ||||
TCD4 | mouse anti-sheep CD4 | RPE | clone 44.38 | MCA2213PE |
TCD8 | mouse anti-bovine CD8 | FITC | clone CC63 | MCA837F |
WC1+γδ | mouse anti-bovine WC1 | FITC | clone CC15 | MCA838F |
CD14 | mouse anti-bovine CD14 | FITC | clone CC-G33 | MCA2678F |
CD83 | mouse anti-human CD83 | FITC | clone HB15e | MCA1582F |
MHCII | mouse anti-bovine MHC class II | FITC | clone IL-A21 | MCA2445F |