Table 1 Quality control characteristics of the qPCR reactions
Gene (assay) | % Reaction efficiency (M) | % Reaction efficiency (S) | R2 (M) | R2 (S) | Dynamic range (M) | Dynamic range (S) | %CV (M) | %CV (S) |
|---|---|---|---|---|---|---|---|---|
RPS7 (Detox A-D) | 104 | 103 | 0.999 | 0.998 | 16.50–31.00 | 16.20–31.00 | 8.89 | 9.52 |
CYP6P3 (Detox A) | 107 | 102 | 0.999 | 0.991 | 25.70–32.92 | 25.96–33.46 | 9.99 | 12.37 |
CYP6M2 (Detox A) | 109 | 103 | 0.998 | 0.996 | 26.39–32.97 | 26.44–34.31 | 9.64 | 10.41 |
CYP9K1 (Detox B) | 105 | 101 | 0.994 | 0.989 | 20.22–31.67 | 20.74–32.02 | 6.50 | 9.64 |
CYP6P4 (Detox B) | 109 | 107 | 0.996 | 0.996 | 25.54–33.94 | 25.83–34.30 | 4.46 | 10.41 |
CYP6Z1 (Detox C) | 99 | 95 | 0.995 | 0.997 | 23.37–33.63 | 23.29–33.29 | 5.54 | 9.99 |
GSTE2 (Detox C) | 100 | 96.1 | 0.999 | 0.998 | 23.25–33.36 | 23.62–33.69 | 12.07 | 16.03 |
CYP6P1 (Detox D) | 97 | 95 | 0.997 | 0.996 | 24.66–34.17 | 24.87–34.27 | 10.37 | 13.55 |
CYP4G16 (Detox D) | 100 | 98 | 0.999 | 0.998 | 19.22–30.30 | 19.52–30.40 | 9.02 | 12.24 |