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Table 1 Quality control characteristics of the qPCR reactions

From: Rapid multiplex gene expression assays for monitoring metabolic resistance in the major malaria vector Anopheles gambiae

Gene (assay) % Reaction efficiency (M) % Reaction efficiency (S) R2 (M) R2 (S) Dynamic range (M) Dynamic range (S) %CV (M) %CV (S)
RPS7 (Detox A-D) 104 103 0.999 0.998 16.50–31.00 16.20–31.00 8.89 9.52
CYP6P3 (Detox A) 107 102 0.999 0.991 25.70–32.92 25.96–33.46 9.99 12.37
CYP6M2 (Detox A) 109 103 0.998 0.996 26.39–32.97 26.44–34.31 9.64 10.41
CYP9K1 (Detox B) 105 101 0.994 0.989 20.22–31.67 20.74–32.02 6.50 9.64
CYP6P4 (Detox B) 109 107 0.996 0.996 25.54–33.94 25.83–34.30 4.46 10.41
CYP6Z1 (Detox C) 99 95 0.995 0.997 23.37–33.63 23.29–33.29 5.54 9.99
GSTE2 (Detox C) 100 96.1 0.999 0.998 23.25–33.36 23.62–33.69 12.07 16.03
CYP6P1 (Detox D) 97 95 0.997 0.996 24.66–34.17 24.87–34.27 10.37 13.55
CYP4G16 (Detox D) 100 98 0.999 0.998 19.22–30.30 19.52–30.40 9.02 12.24
  1. Abbreviations: M, multiplex; S, singleplex; CV, coefficient of variation at the expression units level