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Table 3 Gene expression analysis performed in mosquito lysates by direct qPCR and matched purified eluates after nucleic acid extraction (n = 4 susceptible vs n = 4 resistant). P values were calculated by the pair wise fixed reallocation randomization test

From: Rapid multiplex gene expression assays for monitoring metabolic resistance in the major malaria vector Anopheles gambiae

Gene (Detox assay) Direct qPCR in lysates qPCR in matched nucleic acid extracted eluates
Fold change (95% CI) P-value Fold change (95% CI) P-value
CYP6P3 (A) 4.68 (2.67–8.81) 0.001 4.10 (3.35–5.23) 0.020
CYP6M2 (A) 4.57 (3.18–7.19) 0.002 2.70 (2.18–3.40) 0.022
CYP9K1 (B) 4.66 (3.40–7.70) 0.002 4.45 (4.12–4.80) <0.001
CYP6P4 (B) 2.29 (1.31–3.71) 0.018 1.67 (1.33–2.11) <0.001
CYP6Z1 (C) 2.98 (2.47–3.84) 0.009 1.85 (1.44–2.22) <0.001
GSTE2 (C) 13.6 (9.64–18.4) 0.008 9.36 (7.96–11.9) <0.001
CYP6P1 (D) 1.32 (0.968–1.90) 0.060 1.64 (0.752–3.57) 0.215
CYP4G16 (D) 2.81 (1.61–4.26) 0.008 1.91 (1.45–2.46) <0.001