Table 4 Cost comparison of triplex TaqMan vs singleplex SYBR Green approach. This is a rough cost estimation for measuring the gene expression levels of eight detox genes and one normaliser gene in 12 samples on a standard 96-well plate format
Consumables and reagents | TaqMan triplex RT-qPCR | SYBR Green singleplex RT-qPCR |
|---|---|---|
96-well plastic plate and optical film | 1 × 6.50 € = 6.50 € | 3 × 6.50 € = 19.50 € |
Mastermix | 1 × 25.00 € = 25.00 € | 2.25 × 25.00 € = 56.25 € |
Primers | 1 × 0.50 € = 0.50 € | 2.25 × 0.50 € = 1.13 € |
TaqMan Probes | 1 × 10.00 € = 10.00 € | – |
Total cost | 42.00 € | 76.88 € |