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Table 4 Cost comparison of triplex TaqMan vs singleplex SYBR Green approach. This is a rough cost estimation for measuring the gene expression levels of eight detox genes and one normaliser gene in 12 samples on a standard 96-well plate format

From: Rapid multiplex gene expression assays for monitoring metabolic resistance in the major malaria vector Anopheles gambiae

Consumables and reagents TaqMan triplex RT-qPCR SYBR Green singleplex RT-qPCR
96-well plastic plate and optical film 1 × 6.50 € = 6.50 € 3 × 6.50 € = 19.50 €
Mastermix 1 × 25.00 € = 25.00 € 2.25 × 25.00 € = 56.25 €
Primers 1 × 0.50 € = 0.50 € 2.25 × 0.50 € = 1.13 €
TaqMan Probes 1 × 10.00 € = 10.00 €
Total cost 42.00 € 76.88 €