Fig. 3
From: Mapping of the complement C1q binding site on Trichinella spiralis paramyosin
Determination of the C1q binding fragment of TsPmy within TsPmy1-315aa. a The diagram of fragment design within TsPmy1-315aa. b, c Far-Western blotting showing fragment TsPmy191-315aa binds to C1q. d, e Further narrow-down expression of fragments within TsPmy191-315aa showing that fragment TsPmy226-280aa confers the strong binding ability to human C1q. The fragments were incubated with human C1q (5 μg/ml) and then probed with anti-C1q antibody (1:10,000) or directly probed with anti-His Ab as control for the size of recombinant fragments with His-tag. BSA and Ts87 (with His-tag) served as negative control. Results were repeated three times. Lane M: molecular weight marker