Fig. 5

Inhibition of classical complement activation and complement-mediated hemolysis by peptide P2. a Inhibition of classical complement activation: normal human serum (NHS) was incubated with an increasing dose of peptide P2 (0, 2 and 6 μg), BSA (6 μg), or TsPmy (2 μg), then added to human IgM-coated plates. The C4 deposition was detected with goat anti-human C4 mAb. b Inhibition of complement-mediated hemolysis: C1q was pre-incubated with P2 (0, 0.5 and 1.0 μg), BSA (1 μg), or TsPmy (0.5 μg) before being added to human C1q-deficient serum (C1q D); then, sensitized fresh sheep erythrocytes (SRBC) were added. The percentage of hemolysis was compared with water induced full lysis. BSA, C1q D and NHS alone served as controls. Data are shown as mean ± SD for three independent experiments. *P < 0.05, **P < 0.01. Abbreviation: ns, no significant difference