Fig. 4
Quantitative PCR was performed on microfilariae isolated throughout tetracycline treatment for each study dog. TaqmanĀ® probes were generated for D. immitis 18S and Wolbachia ftsZ genes using FAM and HEX fluorescent probes, respectively. Samples were categorized as negative for the presence of Wolbachia DNA if at least three or more Ct values were ā„ 38 or no DNA was amplified