Fig. 2

Maximum likelihood phylogenetic tree of partial NS5 gene sequences of flavivirus. The maximum likelihood tree was constructed by the GTR + I + G model. The GenBank accession number, virus name, origin, and country/province are noted. The JEV sequences obtained in this study are marked in red. The numbers above each branch represent the bootstrap support for the maximum likelihood, neighbor-joining, and Bayesian analyses, respectively, based on 1000 replicates. The scale-bar indicates 0.1 substitutions per site. Sequences shaded tan represent mosquito-borne flavivirus, those shaded sky blue represent tick-borne flavivirus, those shaded aquamarine represent no-known vector flavivirus, and those shaded khaki represent insect-specific flavivirus. Abbreviations: JEV, Japanese encephalitis virus; SLEV, Santa Louis encephalitis virus; TBEV, tick-borne encephalitis virus; MMLV, Montana myotis leukoencephalitis virus; MODV, Modoc virus; CxFV, Culex flavivirus; QBV, Quang Binh flavivirus; NAKV, Nakiwogo virus; AEFV, Aedes flavivirus; KRV, Kamiti River virus; CFAV, cell fusing agent virus