Table 3 Primer purposes and sequences, and PCR product size for Rhipicephalus microplus VgR silencing experiment
Primer name | Forward primer (5'-3') | Reverse primer (5'-3') | Product size (bp) |
|---|---|---|---|
RmVgR-dsRNA | cgatgaagtcggctgtgatta | accaggcaatgcattcatgg | 361 |
Rm-VgR | attgcgcagatttctcggac | ccgggagttgcacattcatc | 181 |
Rm tub | cgtgccgtatttgttgatc | agattagctgctccgggtg | 91 |
B. bovis rap1-Ex | cacgaggaaggaactaccgatgttga | ccaaggagcttcaacgtacgaggtca | 354 |
B. bovis rap1-In | tcaacaaggtactctatatggctacc | ctaccgagcagaaccttcttcaccat | 291 |