Infectious agent | Test | LMU Munich | Laboklin |
---|---|---|---|
Ehrlichia canis | PCR | Applied Biosystems TaqMan© Real Time PCR [71] | TaqMan© Real Time PCR (in-house test) |
Ab-IFAT | MegaScreen© FLUOEHRLICHIA canis (MegaCor Diagnostik GmbH, Hörbranz, Austria; ≥ 1:40 positive) | MegaFLUO© EHRLICHIA canis (MegaCor Diagnostik GmbH, Hörbranz, Austria; ≥ 1:80 positive) | |
Anaplasma platys | PCR | Applied Biosystems TaqMan© Real Time PCR [72]a | TaqMan© Real Time PCR (in-house test) |
Leishmania infantum | PCR | Applied Biosystems TaqMan© Real Time PCR [73] | TaqMan© Real Time PCR [74] |
Ab-IFAT | Leishmania infantum MON-1 [75]; ≥ 1:64 positive | MegaFLUO© LEISH (MegaCor Diagnostik GmbH, Hörbranz, Austria; › 1:64 positive) | |
Ab-ELISA | - | Civtest© Canis Leishmania (Hipra, Amer, Spain; › 1,1 LE positive) | |
Babesia spp. | PCRb | PCR (18S rRNA) with gel electrophoresis [76]c | PCR (18S rRNA) with gel electrophoresis [77]d |
Babesia canis e | Ab-IFAT | MegaScreen© FLUOBABESIA canis (MegaCor GmbH, Hörbranz, Austria; ≥ 1:64 positive) | MegaFLUO© BABESIA canis (MegaCor GmbH, Hörbranz, Austria; ≥ 1:40 positive) |
Ab-ELISA | – | Babesia ELISA Dog (Afosa, Blankenfelde-Mahlow, Germany; 19 TE positive) | |
Babesia gibsoni | Ab-IFAT | MegaScreen© FLUOBABESIA gibsoni-Testkit (MegaCor GmbH, Hörbranz, Austria; ≥ 1:64 positive) | MegaFLUO© BABESIA gibsoni (MegaCor GmbH, Hörbranz, Austria; ≥ 1:32 positive) |
Babesia spp./Hepatozoon spp. | PCRb | In-house protocol | – |
Hepatozoon canis | PCR | PCR (18S rRNA) with gel electrophoresis [78]f | TaqMan© Real Time PCR (in-house test) |
Dirofilaria spp. | Knottʼs test | Modified Knottʼs test [79] | Modified Knottʼs test [79] |
Microfilariae | PCR | PCR (IST-2) with gel electrophoresis [80]c | TaqMan© Real Time PCR (in-house test)f |
Dirofilaria immitis | Ag-ELISA | Dirochek© Canine Heartworm Antigen Test Kit (Synbiotics Corporation, San Diego, California 92127, US Veterinary License No. 312; Megacor) | FASTest© HW Antigen (MegaCor GmbH, Hörbranz, Austria) |