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Table 2 Number of identified Culicoides to species level by MALDI-TOF mass spectrometry and PCR/sequencing per location in Stara Planina, Serbia. Some individuals identified by more than one method

From: Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia

Species

No. of identified individuals

Location A

Location B

Culicoides group

No. of identifications by MALDI-TOF

No. of identifications by sequencing

C. achrayi

2

2

0

“Others”

0

2

C. circumscriptus

3

3

0

“Others”

1

2

C. clastrieri

1

1

0

“Others”

0

1

C. deltus

5

2

3

“Others”

3

2

C. dewulfi

1

1

0

Obsoletus

0

1

C. fagineus

1

0

1

“Others”

0

1

C. fascipennis

71

49

22

“Others”

16

64

C. festivipennis

11

11

0

“Others”

7

4

C. furcillatus

11

3

8

“Others”

1

10

C. kibunensis

1

1

0

“Others”

0

1

C. lupicaris

31

21

10

Pulicaris

22

14

C. newsteadi

7

7

0

Pulicaris

1

6

C. obsoletus/scoticus

7

2

5

Obsoletus

0

0

C. obsoletus

69

39

30

Obsoletus

61

17

C. pallidicornis

4

4

0

“Others”

2

4

C. parotti

2

2

0

“Others”

0

2

C. picturatus

19

18

1

“Others”

0

19

C. pulicaris

3

2

1

Pulicaris

3

1

C. punctatus

11

11

0

Pulicaris

5

6

C. salinarius

2

2

0

“Others”

0

2

C. scoticus

150

61

89

Obsoletus

142

15

C. simulator

15

12

3

“Others”

0

15

C. subfasciipennis

10

10

0

“Others”

0

10

Culicoides spp.a

155

129

26

 

0

10

Total

592

393

199

 

264

209

  1. aIdentification to Culicoides spp. done as a combination of morphological identification result, indefinite sequencing results (poor identity or ambivalent result) and MALDI- TOF mass spectrometry results. Specimens defined as C. obsoletus/scoticus were identified by morphological identification, while C.obsoletus and C. scoticus were confirmed by molecular methods which enables species identification