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Table 4 Concentrations and growth inhibition effect of thymoquinone combined with diminazene aceturate applied to the cultures of B. bigemina

From: Evaluation of the in vitro and in vivo inhibitory effect of thymoquinone on piroplasm parasites

Concentration (μM)a Fluorescence valueb t-testc
t-value P-value
TQ DA Control DA IC50 Control DA IC50
5.51 0.15 8.81 ± 1.03 287.14 59.50 <0.0001 <0.0001
5.51 0.10 9.13 ± 1.60 213.64 54.50 <0.0001 <0.0001
3.67 0.15 11.40 ± 0.97 293.43 58.36 <0.0001 <0.0001
3.67 0.10 12.09 ± 2.20 162.84 47.52 <0.0001 <0.0001
1.83 0.15 26.17 ± 2.88 120.20 35.62 <0.0001 <0.0001
1.83 0.10 33.46 ± 0.86 281.21 45.44 <0.0001 <0.0001
0.14 0.15 207.37 ±1.54 27.38 56.66 <0.0001 <0.0001
0.14 0.10 223.99 ± 2.27 8.21 58.04 0.0012 <0.0001
Control 235.57 ± 0.90     
DA IC50 106.81 ± 2.66     
  1. aCombinations were based on the calculated IC50 values obtained from the in vitro Babesia fluorescence assay
  2. bEach value was calculated using a fluorescence-based assay in three separate experiments. Each concentration of the drug combination was made in triplicate in each experiment, and the final obtained fluorescence values represent the mean and standard deviation (SD) of three separate experiments after subtracting the background fluorescence for non-parasitized RBCs
  3. ct-test represents the statistically significant differences between the combined drug-treated group and either the control group or the diminazene aceturate-treated group
  4. Abbreviations: TQ thymoquinone, DA diminazene aceturate