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Table 5 Concentrations and growth inhibition effect of thymoquinone combined with diminazene aceturate applied to the cultures of B. caballi

From: Evaluation of the in vitro and in vivo inhibitory effect of thymoquinone on piroplasm parasites

Concentration (μM)a Fluorescence valueb t-testc
t-value P-value
TQ DA Control DA IC50 Control DA IC50
50.25 0.0052 79.25 ± 0.47 450.67 156.58 <0.0001 <0.0001
50.25 0.0035 92.75 ± 1.45 216.27 54.18 <0.0001 <0.0001
33.50 0.0052 104.32 ±1.02 266.65 55.57 <0.0001 <0.0001
33.50 0.0035 113.25 ±1.70 169.62 26.80 <0.0001 <0.0001
16.75 0.0052 115.22 ± 0.83 287.37 45.66 <0.0001 <0.0001
16.75 0.0035 121.89 ± 0.71 302.17 37.62 <0.0001 <0.0001
9.38 0.0052 125.12 ± 0.85 267.38 27.38 <0.0001 <0.0001
9.38 0.0035 162.39 ± 2.60 83.74 14.24 <0.0001 0.0001
Control 292.20 ± 0.67     
DA IC50 140.63 ± 0.49     
  1. aCombinations were based on the calculated IC50 values obtained from the in vitro Babesia fluorescence assay
  2. bEach value was calculated using a fluorescence-based assay in three separate experiments. Each concentration of the drug combination was made in triplicate in each experiment, and the final obtained fluorescence values represent the mean and standard deviation (SD) of three separate experiments after subtracting the background fluorescence for non-parasitized RBCs
  3. ct-test represents the statistically significant differences between the combined drug-treated group and either the control group or the diminazene aceturate-treated group
  4. Abbreviations: TQ thymoquinone, DA diminazene aceturate