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Table 5 Concentrations and growth inhibition effect of thymoquinone combined with diminazene aceturate applied to the cultures of B. caballi

From: Evaluation of the in vitro and in vivo inhibitory effect of thymoquinone on piroplasm parasites

Concentration (μM)a

Fluorescence valueb

t-testc

t-value

P-value

TQ

DA

Control

DA IC50

Control

DA IC50

50.25

0.0052

79.25 ± 0.47

450.67

156.58

<0.0001

<0.0001

50.25

0.0035

92.75 ± 1.45

216.27

54.18

<0.0001

<0.0001

33.50

0.0052

104.32 ±1.02

266.65

55.57

<0.0001

<0.0001

33.50

0.0035

113.25 ±1.70

169.62

26.80

<0.0001

<0.0001

16.75

0.0052

115.22 ± 0.83

287.37

45.66

<0.0001

<0.0001

16.75

0.0035

121.89 ± 0.71

302.17

37.62

<0.0001

<0.0001

9.38

0.0052

125.12 ± 0.85

267.38

27.38

<0.0001

<0.0001

9.38

0.0035

162.39 ± 2.60

83.74

14.24

<0.0001

0.0001

Control

292.20 ± 0.67

    

DA IC50

140.63 ± 0.49

    
  1. aCombinations were based on the calculated IC50 values obtained from the in vitro Babesia fluorescence assay
  2. bEach value was calculated using a fluorescence-based assay in three separate experiments. Each concentration of the drug combination was made in triplicate in each experiment, and the final obtained fluorescence values represent the mean and standard deviation (SD) of three separate experiments after subtracting the background fluorescence for non-parasitized RBCs
  3. ct-test represents the statistically significant differences between the combined drug-treated group and either the control group or the diminazene aceturate-treated group
  4. Abbreviations: TQ thymoquinone, DA diminazene aceturate
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Parasites & Vectors

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