Table 2 Summary of the standard bioinformatics quality checks and cleaning of small RNAs
From: Expression profile of microRNAs in porcine alveolar macrophages after Toxoplasma gondii infection
Library type | Me49-12 | Control-12 | RH-12 | Me49-24 | Control-24 | RH-24 |
|---|---|---|---|---|---|---|
Total reads | 13,132,137 | 13,136,212 | 10,468,948 | 13,959,111 | 14,751,563 | 14,931,863 |
N% > 10% | 1 | 2 | 57 | 69 | 75 | 28 |
Reads with 5-adapter-contamination | 2381 | 716 | 2202 | 2433 | 544 | 2617 |
Reads with ploy A/T/G/C | 44,095 | 21,489 | 41,015 | 29,190 | 11,655 | 60,839 |
Low-quality reads | 82,182 | 78,823 | 22,801 | 2,797,182 | 2,610,836 | 3,360,947 |
3-adapter- null or insert-null reads | 325,730 | 238,506 | 143,336 | 235,442 | 161,513 | 228,911 |
Clean reads (%) | 12,677,748 (96.54) | 12,796,676 (97.42) | 10,259,537 (98.00) | 10,894,795 (78.05) | 11,966,940 (81.12) | 11,278,521 (75.53) |