Fig. 1
Levels of transcription of the GPI-14 gene (a) and concanavalin-A (Con-A) lectin binding profile (b-d) in the wild type and GPI-14 overexpressor L. braziliensis lines. a Levels of GPI-14 mRNA as determined quantitatively (relative to the DNA polymerase Leishmania gene) by real-time RT-PCR. Transcript levels ratio (GPI-14/DNA polymerase gene) ± standard deviations are indicated from three independent experiments. b Flow cytometric analysis of the differential expression of surface carbohydrate in wild-type (LbWT) and GPI14C10 clone (LbGPI14C10) of L. braziliensis using concanavalin-A (Con-A) lectin conjugated to fluorescein isothiocyanate (FITC). c The GPI-14-overexpressor clones were compared to the wild-type L. braziliensis line by the amount of α-D-mannosyl and α-D-glucosyl on its surface using concanavalin-A FITC agglutination profile and a significant difference is seen in the mean intensity of fluorescence (gMIF) in both clones. d The percentage (%) of Con-A FITC labeled parasites was used as a control parameter of efficiency. Data obtained in duplicates from at least three independent experiments were analyzed by Student’s t-test using GraphPad Prism 5.0 software. Statistically significant differences (P < 0.001) between wild type and GPI-14 overexpressor parasites are showed by asterisks (***). Pairwise comparisons: a LbWT vs LbGPI14C4 (t(3) = 21.16, P = 0.0002); LbWT vs LbGPI14C10 (t(4) = 9.130, P = 0.0008); c LbWT vs LbGPI14C4 (t(3) = 25.03, P = 0.0001); LbWT vs LbGPI14C10 (t(3) = 23.92, P = 0.0002)