Skip to main content

Advertisement

Fig. 2 | Parasites & Vectors

Fig. 2

From: Mannosyltransferase (GPI-14) overexpression protects promastigote and amastigote forms of Leishmania braziliensis against trivalent antimony

Fig. 2

SbIII susceptibility assays on the promastigotes and intracellular amastigotes of wild type and GPI-14-overexpressor L. braziliensis clones. a Promastigotes were cultured in the absence or presence of increasing SbIII concentrations (1.2 to 74.9 μM) for 48 h and the percentage of relative growth was determined using a Z1 coulter counter. b, c SbIII susceptibility was evaluated on amastigotes using THP-1-derived human macrophages. THP-1 macrophages infected with L. braziliensis lines were cultured in the absence or presence of increasing SbIII concentrations (12.5 to 200 μM) for 72 h and the percentage of infected macrophages (b) and number of parasites per 100 macrophages (c) was determined. The EC50 (μM) values were calculated for promastigotes (d) and amastigotes (e) of LbWT, LbGPI14C4 and LbGPI14C10 and the fold change in the resistance index of overexpressor clones relative to LbWT was determined. Statistical analysis of the curves was analyzed by Student’s t-test. ‘*’ represents a statistical difference between the wild-type lines and each overexpressor clone (*P < 0.05, **P < 0.01 and ***P < 0.001; see Tables 1 and 2 for details on pairwise comparisons)

Back to article page