Table 1 Details of the qPCR/PCR assays used in the study for the detection of pathogen DNA in flea samples
From: Pathogens in fleas collected from cats and dogs: distribution and prevalence in the UK
Target species (gene) | PCR primer and probe sequences (5'-3') | Product size (bp) | Reference |
|---|---|---|---|
Flea (18S rRNA) | 18S-F: GATCGTACCCACATTACTTG | 1200 | [27] |
18S-R: AAAGAGCTCTCAATCTGTCA | |||
Dipyllidium caninum (28S rRNA) | F: GCATGCAAGTCAAAGGGTCCTACG | 653 | [48] |
R: CACATTCAACGCCCGACTCCTGTAG | |||
Bartonella spp. (ssrA) | F: GCTATGGTAATAAATGGACAATGAAATAA | 299 | [28]a |
R: GGCTTCTGTTGCCAGGTG | |||
FAM-ACCCCGCTTAAACCTGCGACG-BHQ1 | |||
Mycoplasma haemofelis (16S rRNA) | F: GTGCTACAATGGCGAACACA | 80 | [29] |
R: TCCTATCCGAACTGAGACGAA | |||
FAM-TGTGTTGCAAACCAGCGATGGT-BHQ1 | |||
“Candidatus Mycoplasma haemominutum” (16S rRNA) | F: TGATCTATTGTKAAAGGCACTTGCT | 135 | [29] |
R: TTAGCCTCYGGTGTTCCTCAA | |||
FAM-TTCAATGTGTAGCGGTGGAATGCGT-BHQ1 | |||
“Candidatus Mycoplasma turicensis” (16S rRNA) | F: AGAGGCGAAGGCGAAAACT | 138 | [29] |
R: ACGTAAGCTACAACGCCGAAA | |||
FAM-CGTAAACGATGGGTATTAGATGTCGGGAT-BHQ1 |