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Table 2 Comparison of serology results obtained with the different enzyme-linked immuno-sorbent assays used (n = 358) and the foci reduction neutralization test (n = 71)

From: First dengue virus seroprevalence study on Madeira Island after the 2012 outbreak indicates unreported dengue circulation

Serological assays used

Positive samples

n (%)

Negative samples

n (%)

Undetermined

n (%)

n = 358

DENV-1 ED3 dimer ELISA (in-house)

28 (7.8)

330 (92.2)

–

DENV IgG indirect ELISA (Panbio)

32 (8.9)

323 (90.3)

3 (0.8)

DENV IgG capture ELISA (Panbio)

5 (1.4)

349 (97.5)

4 (1.1)

n = 71a

   

DENV-1 ED3 dimer ELISA (in-house)

28 (39.4)

43 (60.6)

–

DENV IgG indirect ELISA (Panbio)

32 (45.1)

36 (50.7)

3 (4.2)

DENV IgG capture ELISA (Panbio)

5 (7.1)

62 (87.3)

4 (5.6)

FRNTb

28 (39.4)

43 (60.6)

–

 DENV-1c

24 (33.8)

47 (66.2)

–

 DENV-2c

5 (7.0)

66 (93.0)

–

 DENV-3c

5 (7.0)

66 (93.0)

–

 DENV-4c

7 (9.9)

64 (90.1)

–

 DENV-1 genotype V

27 (38.0)

44 (62.0)

–

  1. aSubset of additional samples tested by FRNT
  2. bFRNT90 titer ≥ 20
  3. cDengue reference strain
  4. Abbreviations: DENV, dengue virus; ELISA, enzyme-linked immuno-sorbent assay; FRNT, foci reduction neutralization test