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Fig. 4 | Parasites & Vectors

Fig. 4

From: Plant Hsp90 is a novel adjuvant that elicits a strong humoral and cellular immune response against B- and T-cell epitopes of a Toxoplasma gondii SAG1 peptide

Fig. 4

Post-challenge analysis in C57BL/6 immunized mice after T. gondii infection. a Protection against chronic T. gondii infection in C57BL/6 immunized mice. Two weeks after the last immunization, mice were orally challenged with 20 tissue cysts of ME49 T. gondii strain (sub-lethal dose). Thirty days after the infection, mice were sacrificed, and their brains were removed for cyst load determination. Each bar represents the group mean ± SEM. Results represent one of three similar experiments. **P < 0.01, NbHsp90.3-SAG1HC vs NbHsp90.3 group; ****P < 0.0001, NbHsp90.3-SAG1HC vs PBS and SAG1m groups. b, c Antigen-specific antibodies in sera from C57BL/6 immunized mice with chronic T. gondii infection. Thirty days after mice were orally challenged with 20 tissue cysts of ME49 T. gondii strain (sub-lethal dose), serum samples (8 mice per group) were obtained and IgGt was determined by ELISA with TLA (b) or rSAG1m (c) as the bound targets (sera were diluted 1:16,000 and 1:1000, respectively). Each bar represents the group mean ± SEM. Results represent one of two similar experiments. b ***P < 0.001, NbHsp90.3-SAG1HC vs PBS, NbHsp90.3 and SAG1m groups. c **P < 0.01, NbHsp90.3-SAG1HC vs SAG1m group; ***P < 0.001, NbHsp90.3-SAG1HC vs PBS and NbHsp90.3 groups. Pre-immune sera were used as a negative control. Statistical analysis was performed by one-way analysis of variance (ANOVA) using Tukey’s multiple comparisons test

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